Characterizing the genomic variation and population dynamics of Plasmodium falciparum malaria parasites in and around Lake Victoria, Kenya.
Characterizing the genomic variation and population dynamics of Plasmodium falciparum malaria parasites in and around Lake Victoria, Kenya.
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肯尼亚维多利亚湖及其周围地区恶性疟原虫疟疾寄生虫的基因组变异和种群动态特征。
DOI：
10.1038/s41598-021-99192-1
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发表时间：
2021-10-06
影响因子：
4.6
通讯作者：
Clark TG
中科院分区：
文献类型：
--
作者：
Osborne A;Manko E;Takeda M;Kaneko A;Kagaya W;Chan C;Ngara M;Kongere J;Kita K;Campino S;Kaneko O;Gitaka J;Clark TG
描述撒哈拉以南非洲高传播地区恶性疟原虫寄生虫的基因组变异和种群动态对区域疟疾消除运动和根除的长期效力至关重要。全基因组测序（WGS）技术有助于了解恶性疟原虫种群的流行病学和结构变异景观，包括维多利亚湖流域内的种群，这是一个高度传播的地区。在这里，我们提供了一个基线评估的基因组多样性的恶性疟原虫分离在肯尼亚湖地区，其中有稀疏的遗传数据。使用Illumina WGS数据和群体基因组分析，将湖区分离株置于非洲范围内的人群中。我们的分析表明，从维多利亚湖分离的恶性疟原虫在东非寄生虫种群中形成一个簇。这些分离株似乎也有不同的祖先起源，包含来自中非和东非血统的全基因组特征。观察到已知的耐药生物标志物的频率与东非寄生虫种群相似，包括pfap 2 mu基因中的S160 N/T突变，该突变与基于青蒿素的联合疗法的延迟清除有关。总体而言，我们的工作提供了一个恶性疟原虫遗传多样性的第一次评估维多利亚湖流域，一个地区的目标是消除疟疾。
Characterising the genomic variation and population dynamics of Plasmodium falciparum parasites in high transmission regions of Sub-Saharan Africa is crucial to the long-term efficacy of regional malaria elimination campaigns and eradication. Whole-genome sequencing (WGS) technologies can contribute towards understanding the epidemiology and structural variation landscape of P. falciparum populations, including those within the Lake Victoria basin, a region of intense transmission. Here we provide a baseline assessment of the genomic diversity of P. falciparum isolates in the Lake region of Kenya, which has sparse genetic data. Lake region isolates are placed within the context of African-wide populations using Illumina WGS data and population genomic analyses. Our analysis revealed that P. falciparum isolates from Lake Victoria form a cluster within the East African parasite population. These isolates also appear to have distinct ancestral origins, containing genome-wide signatures from both Central and East African lineages. Known drug resistance biomarkers were observed at similar frequencies to those of East African parasite populations, including the S160N/T mutation in the pfap2mu gene, which has been associated with delayed clearance by artemisinin-based combination therapy. Overall, our work provides a first assessment of P. falciparum genetic diversity within the Lake Victoria basin, a region targeting malaria elimination.
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影响因子：
4.6
作者：
Ibrahim, Amy;Benavente, Ernest Diez;Campino, Susana
通讯作者：
Campino, Susana
DOI：
10.1016/j.ijpddr.2017.03.001
发表时间：
2017-08
期刊：
International journal for parasitology. Drugs and drug resistance
影响因子：
--
作者：
Gomes AR;Ravenhall M;Benavente ED;Talman A;Sutherland C;Roper C;Clark TG;Campino S
通讯作者：
Campino S
影响因子：
4.6
作者：
Le Menach, Arnaud;Tatem, Andrew J.;Cohen, Justin M.;Hay, Simon I.;Randell, Heather;Patil, Anand P.;Smith, David L.
通讯作者：
Smith, David L.
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
3.7
作者：
Auburn S;Campino S;Miotto O;Djimde AA;Zongo I;Manske M;Maslen G;Mangano V;Alcock D;MacInnis B;Rockett KA;Clark TG;Doumbo OK;Ouédraogo JB;Kwiatkowski DP
通讯作者：
Kwiatkowski DP