Immune response and protective efficacy of recombinant Enterococcus faecalis displaying dendritic cell-targeting peptide fused with Eimeria tenella 3-1E protein.
Immune response and protective efficacy of recombinant Enterococcus faecalis displaying dendritic cell-targeting peptide fused with Eimeria tenella 3-1E protein.
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DOI：
10.1016/j.psj.2020.03.014
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发表时间：
2020-06
期刊：
影响因子：
4.4
通讯作者：
Ma D
中科院分区：
文献类型：
--
作者：
Chen W;Ma C;Wang D;Li G;Ma D
Avian coccidiosis causes significant economic losses on the global poultry breeding industry. Exploration of new-concept vaccines against coccidiosis has gradually become a research hotspot. In this study, an Enterococcus faecalis strain (MDXEF-1) showing excellent performance isolated from chicken intestinal tract was used as a vector to deliver Eimeria target protein. The plasmid pTX8048-SP-DCpep-NAΔ3-1E-CWA harboring dendritic cell–targeting peptide (DCpep) fusion with Eimeria tenella NAΔ3-1E gene (3-1E protein–coding gene without start codon ATG and terminator codon TAA) was electrotransformed into MDXEF-1 to generate the recombinant bacteria MDXEF-1/pTX8048-SP-DCpep-NAΔ3-1E-CWA in which NAΔ3-1E protein was covalently anchored to the surface of bacteria cells by cell wall anchor (CWA) sequence. The expression of target fusion protein DCpep-NAΔ3-1E-CWA was detected by Western blot. Each chicken was immunized 3 times at 2-wk intervals with live E. faecalis expressing DCpep-NAΔ3-1E fusion protein (DCpep-NAΔ3-1E group), live E. faecalis expressing NAΔ3-1E protein (NAΔ3-1E group), and live E. faecalis containing empty vector only. The 3 immunized groups were then challenged with homologous E. tenella sporulated oocyst after immunizations, and the immune response and protective efficacy in each group were evaluated. The results showed that serum IgG levels, secretory IgA levels in cecal lavage, proportion of CD4+ and CD8α+ cells in peripheral blood, and mRNA expression levels of IL-2 and IFN-γ in the spleen were significantly higher in chickens in the DCpep-NAΔ3-1E group than in chickens of the NAΔ3-1E group (P < 0.05). Oral immunization to chickens with live E. faecalis expressing DCpep-NAΔ3-1E offered more protective efficacy against homologous challenge including significant improved body weight gain, increased oocyst decrease ratio, and reduced average lesion scores in cecum compared with chickens with live E. faecalis expressing NAΔ3-1E protein. These results suggest that recombinant E. faecalis expressing dendritic cell–targeting peptide fusion with E. tenella 3-1E protein could be a potential approach for prevention of Eimeria infection.
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影响因子：
5
作者：
Subramaniam S;Cao D;Tian D;Cao QM;Overend C;Yugo DM;Matzinger SR;Rogers AJ;Heffron CL;Catanzaro N;Kenney SP;Opriessnig T;Huang YW;Labarque G;Wu SQ;Meng XJ
通讯作者：
Meng XJ
影响因子：
4
作者：
Lillehoj, HS
通讯作者：
Lillehoj, HS
影响因子：
2.8
作者：
Giannenas, I.;Tsalie, E.;Tontis, D.
通讯作者：
Tontis, D.
影响因子：
4.4
作者：
Ritzi, Miranda M.;Abdelrahman, Wael;Dalloul, Rami A.
通讯作者：
Dalloul, Rami A.
影响因子：
2.1
作者：
Ma, Chunli;Zhang, Lili;Ma, Dexing
通讯作者：
Ma, Dexing