A synchronized quorum of genetic clocks.
A synchronized quorum of genetic clocks.
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在活细胞中设计具有预测功能的遗传电路是合成生物学不断扩大的领域的一个决定性的焦点。十年前，随着遗传触发开关和振荡器的设计和建造，这一重点被优雅地启动，随后的亮点包括能够产生图案、噪声整形、边缘检测和事件计数的电路。在这里，我们描述了一个具有全球细胞间耦合的工程基因网络，它能够在不断增长的细胞群体中产生同步振荡。使用为不同长度尺度的细胞群体量身定做的微流体装置，我们研究了集体同步特性以及在毫米尺度上发生的时空波。我们使用计算模型来定量地描述观测到的主体振荡的周期和幅度与流速的关系。同步的基因时钟为利用微生物制造具有振荡输出的宏观生物传感器奠定了基础。此外，它还提供了一个特定的模型系统，用于在群体层面上生成对紧急协调行为的机械性描述。
The engineering of genetic circuits with predictive functionality in living cells represents a defining focus of the expanding field of synthetic biology. This focus was elegantly set in motion a decade ago with the design and construction of a genetic toggle switch and an oscillator, with subsequent highlights that have included circuits capable of pattern generation, noise shaping, edge detection, and event counting. Here, we describe an engineered gene network with global intercellular coupling that is capable of generating synchronized oscillations in a growing population of cells. Using microfluidic devices tailored for cellular populations at differing length scales, we investigate the collective synchronization properties along with spatiotemporal waves occurring on millimeter scales. We use computational modeling to quantitatively describe the observed dependence of the period and amplitude of the bulk oscillations on the flow rate. The synchronized genetic clock sets the stage for the use of microbes in the creation of a macroscopic biosensor with an oscillatory output. In addition, it provides a specific model system for the generation of a mechanistic description of emergent coordinated behavior at the colony level.
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影响因子：
3.5
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