Survival of Campylobacter jejuni 11168H in Acanthamoebae castellanii Provides Mechanistic Insight into Host Pathogen Interactions.
Survival of Campylobacter jejuni 11168H in Acanthamoebae castellanii Provides Mechanistic Insight into Host Pathogen Interactions.
复制标题
DOI：
10.3390/microorganisms10101894
复制
发表时间：
2022-09-23
期刊：
影响因子：
4.5
通讯作者：
Wren BW
中科院分区：
文献类型：
--
作者：
Nasher F;Lehri B;Horney MF;Stabler RA;Wren BW
Campylobacter jejuni is the leading cause of bacterial foodborne gastroenteritis worldwide but is rarely transferred between human hosts. Although a recognized microaerophile, the majority of C. jejuni are incapable of growing in an aerobic environment. The persistence and transmission of this pathogen outside its warm-blooded avian and mammalian hosts is poorly understood. Acanthamoebae species are predatory protists and form an important ecological niche with several bacterial species. Here, we investigate the interaction of C. jejuni 11168H and Acanthamoebae castellanii at the single-cell level. We observe that a subpopulation of C. jejuni cells can resist killing by A. castellanii, and non-digested bacteria are exocytosed into the environment where they can persist. In addition, we observe that A. castellanii can harbor C. jejuni 11168H even upon encystment. Transcriptome analyses of C. jejuni interactions revealed similar survival mechanisms when infecting both A. castellanii and warm-blooded hosts. In particular, nitrosative stress defense mechanisms and flagellum function are important as confirmed by mutational analyses of C. jejuni 11168H. This study describes a new host–pathogen interaction for C. jejuni and confirms that amoebae are transient hosts for the persistence, adaptability, and potential transmission of C. jejuni.
登录
查看更多内容
影响因子：
5.6
作者：
Banaś AM;Bocian-Ostrzycka KM;Dunin-Horkawicz S;Ludwiczak J;Wilk P;Orlikowska M;Wyszyńska A;Dąbrowska M;Plichta M;Spodzieja M;Polańska MA;Malinowska A;Jagusztyn-Krynicka EK
通讯作者：
Jagusztyn-Krynicka EK
弯曲杆菌空肠的宿主上皮细胞侵袭：触发或拉链机制？
影响因子：
5.7
作者：
O Cróinín T;Backert S
通讯作者：
Backert S
影响因子：
5
作者：
Alam A;Bröms JE;Kumar R;Sjöstedt A
通讯作者：
Sjöstedt A
影响因子：
4.2
作者：
Jervis AJ;Butler JA;Wren BW;Linton D
通讯作者：
Linton D
影响因子：
2.1
作者：
Axelsson-Olsson, Diana;Olofsson, Jenny;Olsen, Bjorn
通讯作者：
Olsen, Bjorn