Identification and Analysis of the AP2 Subfamily Transcription Factors in the Pecan (Carya illinoinensis).
Identification and Analysis of the AP2 Subfamily Transcription Factors in the Pecan (Carya illinoinensis).
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DOI：
10.3390/ijms222413568
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发表时间：
2021-12-17
影响因子：
5.6
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Yang Z;Jin H;Chen J;Li C;Wang J;Luo J;Wang Z
AP2 转录因子 (TF) 属于 APETALA2/乙烯响应因子 (AP2/ERF) 超家族，调节植物生长和发育的各种生物过程以及对生物和非生物胁迫的响应。然而，针对山核桃（Carya illinoinensis）AP2亚家族转录因子的全基因组研究却鲜有报道。在本文中，我们通过全基因组搜索从山核桃中鉴定出30个AP2亚家族基因，它们在山核桃染色体上的分布不均匀。然后，进一步分析了系统发育树、基因结构和保守基序。将30个AP2基因分为euAP2、euANT和basalANT三个分支。此外，顺式作用元件分析表明，CiAP2启动子中存在多种光响应元件、植物激素响应元件和非生物胁迫响应元件。此外，qPCR 分析表明，聚集在一起的基因在 euAP2 和 basalANT 进化枝中通常具有相似的表达模式，而 euANT 进化枝中的表达模式差异很大。在山核桃果实发育过程中，CiAP2-5、CiANT1和CiANT2具有相似的表达模式，并且它们的表达水平随着果实发育而降低。 CiANT5 在发育中的果实中表现出最高的表达水平。亚细胞定位和转录激活活性测定表明，CiANT5位于细胞核中，作为具有转录激活活性的转录因子。这些结果有助于全面了解山核桃AP2亚家族转录因子，为进一步研究山核桃AP2家族基因的功能奠定基础。
The AP2 transcriptional factors (TFs) belong to the APETALA2/ ethylene-responsive factor (AP2/ERF) superfamily and regulate various biological processes of plant growth and development, as well as response to biotic and abiotic stresses. However, genome-wide research on the AP2 subfamily TFs in the pecan (Carya illinoinensis) is rarely reported. In this paper, we identify 30 AP2 subfamily genes from pecans through a genome-wide search, and they were unevenly distributed on the pecan chromosomes. Then, a phylogenetic tree, gene structure and conserved motifs were further analyzed. The 30 AP2 genes were divided into euAP2, euANT and basalANT three clades. Moreover, the cis-acting elements analysis showed many light responsive elements, plant hormone-responsive elements and abiotic stress responsive elements are found in CiAP2 promoters. Furthermore, a qPCR analysis showed that genes clustered together usually shared similar expression patterns in euAP2 and basalANT clades, while the expression pattern in the euANT clade varied greatly. In developing pecan fruits, CiAP2-5, CiANT1 and CiANT2 shared similar expression patterns, and their expression levels decreased with fruit development. CiANT5 displayed the highest expression levels in developing fruits. The subcellular localization and transcriptional activation activity assay demonstrated that CiANT5 is located in the nucleus and functions as a transcription factor with transcriptional activation activity. These results help to comprehensively understand the pecan AP2 subfamily TFs and lay the foundation for further functional research on pecan AP2 family genes.
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