ATHK1 acts downstream of hydrogen peroxide to mediate ABA signaling through regulation of calcium channel activity in Arabidopsis guard cells

ATHK1 acts downstream of hydrogen peroxide to mediate ABA signaling through regulation of calcium channel activity in Arabidopsis guard cells
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ATHK1 作用于过氧化氢下游,通过调节拟南芥保卫细胞中的钙通道活性来介导 ABA 信号传导

DOI:
10.1007/s11434-012-5207-y
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发表时间:
2013-01
影响因子:
--
通讯作者:
Lü, D.
Lü, D.
中科院分区:
--
文献类型:
--
作者:
Lu D.;Wang W.;Miao C.;Lü, D.

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作为进化过程的一部分,植物逐渐发展出耐受环境缺水的能力。脱落酸 (ABA) 在干旱和渗透胁迫期间发挥着关键作用。几种组氨酸蛋白激酶被认为是植物对缺水的适应性反应中的渗透传感器或调节剂。在这项研究中,我们报道了先前被证明具有渗透调节作用的 ATHK1 参与拟南芥中 ABA 诱导的气孔信号传导。 ATHK1表达无效的突变体无法在保卫细胞中传递正常的ABA反应,包括诱导气孔关闭、产生过氧化氢和激活钙内流。此外,膜片钳和共聚焦分析表明,ATHK1 可能通过调节拟南芥保卫细胞中的钙通道活性和钙振荡,在 ABA 诱导的气孔关闭中发挥过氧化氢下游的作用。
Plants gradually develop their ability to tolerate environmental water deficit as part of the evolutionary process. Abscisic acid (ABA) plays a critical role during drought and osmotic stress. Several histidine protein kinases are regarded as osmotic sensors or regulators in the adaptive response of plants to water deficit. In this study, we report that ATHK1, which was previously shown to function as an osmotic regulator, is involved in ABA-induced stomatal signaling in Arabidopsis. Mutants null for ATHK1 expression were unable to transmit normal ABA responses in guard cells, including inducing stomatal closure, producing hydrogen peroxide and activating calcium influx. Moreover, patch clamp and confocal analysis demonstrated that ATHK1 may function downstream of hydrogen peroxide in ABA-induced stomatal closure, by regulating calcium channel activity and calcium oscillation in Arabidopsis guard cells.
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