Safety Evaluation and Probiotic Potency Screening of Akkermansia muciniphila Strains Isolated from Human Feces and Breast Milk.
Safety Evaluation and Probiotic Potency Screening of Akkermansia muciniphila Strains Isolated from Human Feces and Breast Milk.
复制标题
从人类粪便和母乳中分离的阿克曼氏菌菌株的安全性评价和益生菌效力筛选
DOI：
10.1128/spectrum.03361-22
复制
发表时间：
2023-02-14
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究从不同的人粪便标本中分离到30株嗜粘蛋白阿克曼氏菌，并首次分离到一株嗜粘蛋白阿克曼氏菌。来自人类母乳的嗜粘蛋白菌株。这种分离证实了人类母乳中微生物的存在，这表明A。嗜粘蛋白菌可以从母亲垂直繁殖到婴儿，并参与早期肠道微生物组的形成。摘要嗜粘蛋白阿克曼氏菌因其调节疾病的巨大潜力而被认为是下一代益生菌。共分离出31株A.来自健康人的粪便或母乳的嗜粘蛋白菌。在基因组测序、组装和分析之后，我们选择了六个菌株（AM 01至AM 06）用于进一步探索。我们首先分析了它们的一般特征，包括形态描述、生长特性和生理生化特性，然后确定了它们的遗传特征，包括GC含量、推定的毒力因子和抗生素抗性基因。我们接下来研究了这些菌株对人工胃液和肠液以及胆盐的耐受性，以评估它们在消化道中的存活潜力。并对分离菌株进行耐药基因分析，进行药敏试验。此外，我们还研究了两个菌株（AM 02和AM 06）在小鼠体内的遗传稳定性和急性毒性。最后，在正常小鼠和裸鼠中评价AM 06的安全性。AM 06对pH值变化具有较强的适应性。由于AM 02和AM 03对抗生素的耐药性比AM 01和AM 04对AM 06的耐药性更强，其潜在的临床应用可能受到限制。AM 02和AM 06在正常小鼠中均具有遗传和表型稳定性和安全性，而AM 06在裸鼠中是安全的。综合考虑，AM 06是一个安全的A。在分离的菌株中显示出作为益生菌菌株的巨大潜力。在本研究中，我们从不同的人类粪便样品中分离了30株嗜粘蛋白阿克曼氏菌，并首次分离到一株嗜粘蛋白阿克曼氏菌。来自人类母乳的嗜粘蛋白菌株。这种分离证实了人类母乳中微生物的存在，这表明A。嗜粘蛋白菌可以从母亲垂直繁殖到婴儿，并参与早期肠道微生物组的形成。然后，我们系统地评估了这种A.根据FAO/WHO的建议，我们证实从母乳中分离的AM 06菌株没有毒力因子，并且对正常小鼠和裸鼠都是遗传稳定的和非致病性的。此外，其对pH变化和胆汁盐的耐受性表明其具有理想的益生菌特性。因此，我们建议A.嗜粘蛋白菌作为益生菌候选物是安全的。
In this study, we isolated 30 strains of Akkermansia muciniphila from different samples of human feces, and for the first time we isolated an A. muciniphila strain from human breast milk. This isolation verified the existence of microbes in human breast milk, which suggests that A. muciniphila can be vertically propagated from mother to infant and participates in the formation of the early gut microbiome. ABSTRACT Akkermansia muciniphila is considered a next-generation probiotic because of its immense potential to regulate disorders. We isolated 31 strains of A. muciniphila from feces or breast milk of healthy people. After genome sequencing, assembly, and analysis, we selected six strains (AM01 to AM06) for further exploration. We first analyzed their general characteristics, including morphological description, growth characteristics, and physiological and biochemical characteristics, and then confirmed their genetic characteristics, including GC content, putative virulence factors, and antibiotic resistance genes. We next investigated the tolerance of these strains to artificial gastric and intestinal fluids and bile salts to evaluate their survival potential in the digestive tract. Drug sensitivity tests were also conducted based on the analysis of the antibiotic resistance genes of these strains. Furthermore, we examined the genetic stability and acute toxicity of two strains (AM02 and AM06) in mice. Finally, the safety of AM06 was evaluated in normal mice and nude mice. AM06 exhibited adaptability to pH changes. Since AM02 and AM03 showed more resistance to antibiotics than AM01 and AM04 to AM06, their potential clinical application may be limited. Both AM02 and AM06 were genetically and phenotypically stable and safe in normal mice, and AM06 was safe in nude mice. Considering all this together, AM06 is a safe A. muciniphila strain and exhibits a great potential for use as a probiotic strain among the isolated strains. IMPORTANCE In this study, we isolated 30 strains of Akkermansia muciniphila from different samples of human feces, and for the first time we isolated an A. muciniphila strain from human breast milk. This isolation verified the existence of microbes in human breast milk, which suggests that A. muciniphila can be vertically propagated from mother to infant and participates in the formation of the early gut microbiome. We then systematically evaluated the potential for use as a probiotic of this A. muciniphila strains according to the FAO/WHO recommendation. We confirmed that the AM06 strain isolated from breast milk has no virulence factors and is genetically stable and nonpathogenic for both normal mice and nude mice. Moreover, its tolerance to pH changes and bile salts indicates its desirable probiotic properties. Thus, we propose that the AM06 strain of A. muciniphila is safe for use as a probiotic candidate.
登录
查看更多内容
影响因子：
24.5
作者：
Cani PD
通讯作者：
Cani PD
影响因子：
12.8
作者：
Chelakkot C;Choi Y;Kim DK;Park HT;Ghim J;Kwon Y;Jeon J;Kim MS;Jee YK;Gho YS;Park HS;Kim YK;Ryu SH
通讯作者：
Ryu SH
影响因子：
4.2
作者：
Hou, Fengyi;Chang, Yuxiao;Bi, Yujing
通讯作者：
Bi, Yujing
影响因子：
82.9
作者：
Depommier, Clara;Everard, Amandine;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.