Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation.
Unique uterine localization and regulation may differentiate LPA3 from other lysophospholipid receptors for its role in embryo implantation.
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DOI：
10.1016/j.fertnstert.2011.02.024
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发表时间：
2011-05
影响因子：
6.7
通讯作者：
Chun J
中科院分区：
文献类型：
--
作者：
Ye X;Herr DR;Diao H;Rivera R;Chun J
确定LPA3与其他9种溶血磷脂(LP)受体在胚胎着床中的区别因素。实验小鼠模型。研究所/大学研究实验室。野生型、Lpar3(−/−)、Lpar1(−/−)Lpar2(−/−)和S1Pr2(−/−)S1pr3(−/−)小鼠。卵巢切除术。妊娠4.5天蓝色染料注射用于确定植入部位。实时荧光定量聚合酶链式反应检测子宫全层和子宫层的基因表达原位杂交技术检测孕酮(P)诱导的子宫腔上皮(LE)Lpar3的表达Lpar1(−/−)、Lpar2(−/−)和S1Pr2(−/−)S1pr3(−/−)雌性胚胎着床正常。Lpar3在着床前子宫中表达最强，其余9种LP受体在着床期子宫中呈结构性表达。空间定位显示Lpar3主要表达于LE，其余Lp受体广泛表达于LE、间质和肌层。去卵巢子宫的激素调节表明，P可上调Lpar3的表达，但对其余9种LP受体无影响，17β-雌二醇(E_2)可下调包括Lpar3在内的大多数LP受体的表达。孕酮对LPA3的定位和上调使LPA3有别于其他9种Lp受体，可能是LPA3在胚胎着床中的重要作用。
To determine factors differentiating LPA3 from other nine lysophospholipid (LP) receptors for its role in embryo implantation. Experimental mouse models. Institute/University research laboratories. Wild type, Lpar3(−/−), Lpar1(−/−)Lpar2(−/−), and S1pr2(−/−)S1pr3(−/−) mice. Ovariectomy. Blue dye injection for determining implantation sites on gestation day 4.5. Realtime PCR for measuring gene expression in whole uterus and separated uterine layers. In situ hybridization for detecting progesterone (P)-induced Lpar3 expression in the uterine luminal epithelium (LE). Normal implantation was observed in Lpar1(−/−)Lpar2(−/−) and S1pr2(−/−)S1pr3(−/−) females. Temporal expression showed peak expression of Lpar3 in the preimplantation uterus and constitutive expression of the other nine LP receptors in the peri-implantation uterus. Spatial localization revealed main expression of Lpar3 in the LE and broad expression of the remaining LP receptors in all three main uterine layers: LE, stromal, and myometrial layers. Hormonal regulation in ovariectomized uterus indicated upregulation of Lpar3 but downregulation or no effect of the remaining nine LP receptors by P, and downregulation of most LP receptors, including Lpar3, by 17β-estradiol (E2). LE localization and upregulation by progesterone differentiates LPA3 from the other nine LP receptors and may underlie its essential role in embryo implantation.
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影响因子：
6.7
作者：
Diao, Honglu;Paria, Bibhash C.;Xiao, Shuo;Ye, Xiaoqin
通讯作者：
Ye, Xiaoqin
DOI：
10.1083/jcb.135.4.1071
发表时间：
1996-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hecht JH;Weiner JA;Post SR;Chun J
通讯作者：
Chun J
DOI：
10.1073/pnas.97.24.13384
发表时间：
2000-11-21
影响因子：
11.1
作者：
Contos, JJA;Fukushima, N;Chun, J
通讯作者：
Chun, J
影响因子：
6.1
作者：
Carver, J;Martin, K;Mardon, H
通讯作者：
Mardon, H
影响因子：
5.3
作者：
Herr, Deron R.;Grillet, Nicolas;Chun, Jerold
通讯作者：
Chun, Jerold