Temporal expression pattern of progesterone receptor in the uterine luminal epithelium suggests its requirement during early events of implantation.
Temporal expression pattern of progesterone receptor in the uterine luminal epithelium suggests its requirement during early events of implantation.
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DOI：
10.1016/j.fertnstert.2011.01.160
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发表时间：
2011-05
影响因子：
6.7
通讯作者：
Ye, Xiaoqin
中科院分区：
文献类型：
--
作者：
Diao, Honglu;Paria, Bibhash C.;Xiao, Shuo;Ye, Xiaoqin
确定PR从子宫腔上皮（LE）消失的确切时间，以帮助了解胚胎着床过程中LE中PR动态表达的意义。实验啮齿动物模型。大学研究实验室。老鼠和仓鼠假孕和人工脱卵。蓝色染料注射用于检测胚胎附着;免疫组织化学、免疫荧光和原位杂交用于检测基因表达。在小鼠中最初检测到蓝色染料反应后6小时（第三天22：00小时），PR仍在LE中表达。PR首先从着床部位的LE细胞中消失，随后在第4天06：00时从整个LE层中消失，此时子宫间质蜕膜化已变得明显。PR在第四天11：00小时假孕小鼠的LE中保持高表达，但在第四天06：00小时人工蜕膜化假孕小鼠的整个LE层中PR消失。1)PR从LE消失后，植入已经开始，并在组织学蜕膜化表现出来，这表明PR在LE中的持续表达的积极作用的初始植入过程中;和2）PR表达的消失在LE是由子宫因子（S）胚胎附着时产生的调节。
To determine the precise timing of PR disappearing from the uterine luminal epithelium (LE) to help understand the significance of the dynamic PR expression in the LE during embryo implantation. Experimental rodent models. University research laboratories. Mice and hamsters. Pseudopregnancy and artificial decidaulization. Blue dye injection for detecting embryo attachment; immunohistochemistry, immunofluorescence, and in situ hybridization for detecting gene expression. PR remains expressed in the LE up to 6 hours after the initial detection of blue dye reaction in mice (day three 22:00 hours). PR disappears first from LE cells at the implantation site and subsequently from the entire LE layer by day four 06:00 hours when uterine stromal decidualization has become obvious. PR remains highly expressed in the LE of day four 11:00 hours pseudopregnant mice, but it disappears from the entire LE layer by day four 06:00 hours of artificially decidualized pseudopregnant mice. 1) PR disappears from the LE after implantation has initiated and before the histological decidualization is manifested, suggesting an active role of continued PR expression in the LE for the initial implantation process; and 2) the disappearance of PR expression in the LE is regulated by uterine factor(s) produced upon embryo attachment.
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影响因子：
--
作者：
Cheon, YP;Li, QX;Bagchi, MK
通讯作者：
Bagchi, MK
DOI：
10.1073/pnas.1732707100
发表时间：
2003-08-19
影响因子：
11.1
作者：
Mulac-Jericevic, B;Lydon, JP;Conneely, OM
通讯作者：
Conneely, OM
影响因子：
3.6
作者：
Kurita, T;Lee, K;Cunha, GR
通讯作者：
Cunha, GR
影响因子：
4.8
作者：
Jeong, JW;Lee, KY;DeMayo, FJ
通讯作者：
DeMayo, FJ
影响因子：
5.8
作者：
LESSEY, BA;KILLAM, AP;MCCARTY, KS
通讯作者：
MCCARTY, KS