Characteristics of highly flexible PDMS membranes for long-term mechanostimulation of biological tissue.
Characteristics of highly flexible PDMS membranes for long-term mechanostimulation of biological tissue.
复制标题
用于生物组织长期机械刺激的高度柔性 PDMS 膜的特性。
DOI：
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
J. Guttmann
中科院分区：
文献类型：
--
作者：
C. Armbruster;Matthias Schneider;S. Schumann;K. Gamerdinger;M. Cuevas;S. Rausch;G. Baaken;J. Guttmann
软体生物组织的力学特性测量在力学生物学中仍然是一项具有挑战性的任务。最近，我们提出了一种生物反应器，用于同时进行力学刺激和分析软生物组织样品的力学特性。在这种生物反应器中，样品通过柔性膜的偏转被拉伸。研究发现，使用高度柔顺的膜增加了测量的准确性。在这里，我们描述了薄而柔性的聚二甲基硅氧烷（PDMS）膜的生产过程和特性，旨在提高我们的生物反应器的信噪比。采用自旋涂覆工艺，将双组分弹性体聚合成PDMS膜。树脂组分配比、旋转时间和纺丝速度对膜力学性能的影响均与膜力学有关。膜的内径为22毫米，厚度为33至36微米均匀轮廓。分离的大鼠膈肌作为生物组织样本。在24小时的机械刺激中，膜的机械性能保持不变。相反，发现了膜片的时间和应变依赖的力学性能。
Measurement of mechanical properties of soft biological tissue remains a challenging task in mechanobiology. Recently, we presented a bioreactor for simultaneous mechanostimulation and analysis of the mechanical properties of soft biological tissue samples. In this bioreactor, the sample is stretched via deflection of a flexible membrane. It was found that the use of highly compliant membranes increases accuracy of measurements. Here, we describe the production process and characteristics of thin and flexible membranes of polydimethylsiloxane (PDMS) designed to improve the signal-to-noise ratio of our bioreactor. By a spin-coating process, PDMS membranes were built by polymerization of a two component elastomer. The influence of resin components proportion, rotation duration, and speed of the spinning were related to the membrane mechanics. Membranes of 22 mm inner diameter and 33 to 36 microm thickness at homogeneous profiles were produced. Isolated rat diaphragms served as biological tissue samples. Mechanical properties of the membranes remained constant during 24 h of mechanostimulation. In contrast, time- and strain-dependent mechanical properties of the diaphragms were found.
机械应变增加体外肺成纤维细胞中 I 型胶原蛋白的表达。
DOI：
10.1152/jappl.2000.88.1.203
发表时间：
2000
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
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作者：
Breen,EC
通讯作者：
Breen,EC
影响因子：
2.4
作者：
Yang, GG;Crawford, RC;Wang, JHC
通讯作者：
Wang, JHC