Quantification of Chemical Uptake into the Skin by Vibrational Spectroscopies and Stratum Corneum Sampling.
Quantification of Chemical Uptake into the Skin by Vibrational Spectroscopies and Stratum Corneum Sampling.
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DOI：
10.1021/acs.molpharmaceut.2c01109
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发表时间：
2023-05-01
影响因子：
4.9
通讯作者：
Guy, Richard H.
中科院分区：
文献类型：
--
作者：
Tabosa, M. Alice Maciel;Vitry, Pauline;Zarmpi, Panagiota;Bunge, Annette L.;Belsey, Natalie A.;Tsikritsis, Dimitrios;Woodman, Timothy J.;White, K. A. Jane;Delgado-Charro, M. Begona;Guy, Richard H.
关键词：
在使用复杂的局部产品后，要评估打算在皮肤内作用的药物的生物利用度，需要应用多种实验工具，这些工具必须是定量的、经过验证的，并且在理想情况下也是最终足够微创的，以允许在体内使用。这里的目的是表明红外(IR)和拉曼光谱都可以评估化学物质进入角质层(SC)的摄取情况，这与胶带剥离法对其进行定量直接相关。实验使用离体猪皮肤进行，并测量了应用时间和配方组成对SC中化学处置的影响。每条胶带上去除的SC中的化学物质的量是根据特定分子振动的红外和拉曼信号强度分别测量的，在皮肤处于光谱沉默的频率下，并通过随后的常规提取和层析分析来确定。光谱结果与胶条上的化学定量结果具有良好的相关性，并通过不同的测量技术清楚地描述了较长使用时间和不同载体使用的效果。基于这一初步研究，现在可以探索光谱学方法(特别是拉曼光谱)在多大程度上可以用于询问皮肤更深层次和SC以外的化学处置。
Evaluation of the bioavailability of drugs intended to act within the skin following the application of complex topical products requires the application of multiple experimental tools, which must be quantitative, validated, and, ideally and ultimately, sufficiently minimally invasive to permit use in vivo. The objective here is to show that both infrared (IR) and Raman spectroscopies can assess the uptake of a chemical into the stratum corneum (SC) that correlates directly with its quantification by the adhesive tape-stripping method. Experiments were performed ex vivo using excised porcine skin and measured chemical disposition in the SC as functions of application time and formulation composition. The quantity of chemicals in the SC removed on each tape-strip was determined from the individually measured IR and Raman signal intensities of a specific molecular vibration at a frequency where the skin is spectroscopically silent and by a subsequent conventional extraction and chromatographic analysis. Correlations between the spectroscopic results and the chemical quantification on the tape-strips were good, and the effects of longer application times and the use of different vehicles were clearly delineated by the different measurement techniques. Based on this initial investigation, it is now possible to explore the extent to which the spectroscopic approach (and Raman in particular) may be used to interrogate chemical disposition deeper in the skin and beyond the SC.
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DOI：
10.1208/s12248-021-00571-3
发表时间：
2021-03-29
期刊：
The AAPS journal
影响因子：
--
作者：
Hoppel M;Tabosa MAM;Bunge AL;Delgado-Charro MB;Guy RH
通讯作者：
Guy RH
影响因子：
5.8
作者：
Cordery SF;Pensado A;Chiu WS;Shehab MZ;Bunge AL;Delgado-Charro MB;Guy RH
通讯作者：
Guy RH
影响因子：
4.9
作者：
Pensado, Andrea;Hattam, Laura;Delgado-Charro, M. Begona
通讯作者：
Delgado-Charro, M. Begona
影响因子：
3.7
作者：
Herkenne, Christophe;Alberti, Ingo;Naik, Aarti;Kalia, Yogeshvar N.;Mathy, Franois-Xavier;Preat, Veronique;Guy, Richard H.
通讯作者：
Guy, Richard H.
影响因子：
6.5
作者：
ANDERSON, RL;CASSIDY, JM
通讯作者：
CASSIDY, JM