Effects of Estradiol and Progesterone-Induced Intracellular Calcium Fluxes on Toxoplasma gondii Gliding, Microneme Secretion, and Egress.
Effects of Estradiol and Progesterone-Induced Intracellular Calcium Fluxes on Toxoplasma gondii Gliding, Microneme Secretion, and Egress.
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雌二醇和黄体酮诱导的细胞内钙流对弓形虫滑动、微线体分泌和排出的影响
DOI：
10.3389/fmicb.2018.01266
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发表时间：
2018
影响因子：
5.2
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Zhang X;Zhang H;Fu Y;Liu J;Liu Q
研究表明，雌激素在脊椎动物的繁殖和新陈代谢中起着关键作用，但类固醇对弓形虫的影响还没有引起足够的重视。我们的数据表明，雌二醇和孕酮诱导了寄生性胞浆内钙离子的流动。这一过程需要雌激素进入弓形虫的细胞质，cGMP依赖的蛋白激酶G(PKG)和磷脂酶C(PI-PLC)成为控制寄生细胞内钙信号的重要因素。细胞内钙离子主要是从酸性细胞器中动员出来的，受雌二醇的调节。此外，我们先前的研究表明，胞内钙离子略微增加了MIC2蛋白的分泌，促进了寄生虫的滑行和排泄，从而增强了弓形虫的致病性。我们随后确定，黄体酮调节的钙离子的主要来源是中性储备物。与雌二醇的研究结果相反，孕酮减少了MIC2蛋白的分泌，并抑制了寄生虫的滑动运动，这可能降低了它们的致病性。此外，与哺乳动物不同，雌二醇和孕酮对弓形虫体内一氧化氮(NO)或活性氧(ROS)的产生没有影响。
Research has shown that estrogen is present and plays a critical role in vertebrate reproduction and metabolism, but the influence of steroids on Toxoplasma gondii has received less attention. Our data showed that estradiol and progesterone induced parasitic cytosolic Ca2+ fluxes. This process required estrogen to enter the cytoplasm of T. gondii, and cGMP-dependent protein kinase G (PKG) and phosphoinositide-phospholipase C (PI-PLC) emerged as important factors controlling parasitic intracellular (IC) Ca2+ signals. Cytosolic Ca2+, which is regulated by estradiol, was mostly mobilized from acidic organelles. Moreover, cytosolic Ca2+ slightly increased MIC2 protein secretion and promoted the gliding motility and egress of parasites, thus enhancing the pathogenicity of T. gondii, as shown in our previous research. We subsequently determined that the main source of Ca2+ regulated by progesterone was a neutral store. In contrast to the findings of estradiol, progesterone reduced MIC2 protein secretion and inhibited the gliding motility of parasites, which may decrease their pathogenicity. Additionally, unlike in mammals, estradiol and progesterone had no effect on nitric oxide (NO) or reactive oxygen species (ROS) production in T. gondii.
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影响因子：
3.4
作者：
Raabe AC;Wengelnik K;Billker O;Vial HJ
通讯作者：
Vial HJ
影响因子：
6.7
作者：
Collins CR;Hackett F;Strath M;Penzo M;Withers-Martinez C;Baker DA;Blackman MJ
通讯作者：
Blackman MJ
影响因子：
3.3
作者：
Håkansson, S;Morisaki, H;Sibley, LD
通讯作者：
Sibley, LD
DOI：
10.1073/pnas.94.25.13955
发表时间：
1997-12-09
影响因子：
11.1
作者：
Khan, IA;Schwartzman, JD;Kasper, LH
通讯作者：
Kasper, LH
影响因子：
3.4
作者：
Morlon-Guyot J;Berry L;Chen CT;Gubbels MJ;Lebrun M;Daher W
通讯作者：
Daher W