Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements
Characterization of the aggregated pattern of CHAPS using solvent paramagnetic relaxation enhancements
复制标题
使用溶剂顺磁弛豫增强表征 CHAPS 的聚集模式
DOI：
10.1016/j.colsurfa.2018.07.008
复制
发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Liu Maili
中科院分区：
文献类型：
--
作者：
Zhang Liang;Chai Xin;Sun Peng;Zhu Qinjun;Zhang Xu;Liu Maili
CHAPS是一种典型的两性离子洗涤剂，广泛应用于蛋白质纯化。它有两种特殊的聚集状态，其聚集模式仍然存在争议和不确定。本文通过1H溶剂顺磁驰豫增强(SPRE)实验，分析了CHAPS的聚集态。结果表明，CHAPS的头部基团的1HsPRE比尾基团的小得多，当CHAPS的浓度高于CMC2时，两者同时变小。此外，还发现在两种胶束状态(M1和M2)中，甾体环上的质子的sPRE非常相似。这些结果表明，CHAPS的胶束核主要是由其类固醇头部无序聚集形成的。另一方面，当CHAPS的浓度高于CMC2时，最有可能发生从单层胶束到双层胶束的转变。M2状态的胶束也是由部分无序的初级胶束聚集而成。此外，饱和转移差实验表明，在M2状态下，水和类固醇头部质子附近的OH之间的表观相干转移速率较慢，这表明CHAPS在M1状态下松散聚集，但在M2状态下聚集得更紧密，这可能是由于附近CHAPS分子中的类固醇基团之间的疏水相互作用增加，以及疏水侧的OH与不同分子亲水侧的SO3−1、CO或HN之间的氢键，从而挤出水并稳定胶束所致。
CHAPS is a typical zwitterionic detergent widely used in protein purification. It has two peculiar aggregation states, whose aggregation patterns are still controversial and uncertain. In this paper, we analyzed the aggregation pattern of CHAPS by1H solvent Paramagnetic Relaxation Enhancement (sPRE) experiment. It was found that the1H sPREs of head groups of CHAPS are much smaller than those of the tail groups, and all of them become smaller simultaneously when the concentration of CHAPS is above CMC2. In addition, the sPREs of those protons in the steroid ring were found to be very similar to each other in both micellar states (M1 and M2). These results indicated that the micellar core of CHAPS is mainly formed by its steroid head which aggregate disorderly. On the other hand, a transition from single to double layer micelle is most likely to happen when the concentration of CHAPS is above CMC2. The micelle in M2 state is formed by the aggregation of the partially disordered primary ones as well. Furthermore, the STD (Saturation Transfer Difference) experiment showed that the apparent coherence transfer rates between water and those OH near protons at the steroid head are slower in M2 state, revealing that the CHAPS aggregate loosely in M1 state, but more tightly in M2 state, which may be driven by the increased hydrophobic interactions between the steroid groups of nearby CHAPS molecules, as well as the hydrogen bond between OH in the hydrophobic side and SO3−1, CO, or HN in the hydrophilic side of different molecules, which consequently extrude the water and stabilize the micelle.
登录
查看更多内容
影响因子：
3.5
作者：
Chattopadhyay, A;Harikumar, KG
通讯作者：
Harikumar, KG
影响因子：
3.3
作者：
Lipfert, Jan;Columbus, Linda;Doniach, Sebastian
通讯作者：
Doniach, Sebastian
影响因子：
2.7
作者：
A. Dingley;J. Mackay;B. Chapman;M. Morris;P. Kuchel;B. Hambly;G. King
通讯作者：
A. Dingley;J. Mackay;B. Chapman;M. Morris;P. Kuchel;B. Hambly;G. King
DOI：
10.1016/0166-6622(90)80030-8
发表时间：
1990
期刊：
Colloids and Surfaces
影响因子：
--
作者：
Zhisheng Gao;J. Kwak;R. Labonté;D. Marangoni;R. Wasylishen
通讯作者：
Zhisheng Gao;J. Kwak;R. Labonté;D. Marangoni;R. Wasylishen
DOI：
10.1021/jp108694v
发表时间：
2011-03
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Xianguo Qin;Maili Liu;Xu Zhang;Daiwen Yang
通讯作者：
Xianguo Qin;Maili Liu;Xu Zhang;Daiwen Yang