A comparative study of ammonia-oxidizing archaea and bacteria in acidic and alkaline purple soils
A comparative study of ammonia-oxidizing archaea and bacteria in acidic and alkaline purple soils
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酸性和碱性紫色土中氨氧化古菌和细菌的比较研究
DOI：
10.1007/s13213-015-1143-9
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发表时间：
2016-06
影响因子：
3
通讯作者：
Xie De-Ti
中科院分区：
文献类型：
--
作者：
Zhou Zhi-Feng;Wang Ming-Xia;Liu Wan-Lu;Li Zhen-Lun;Luo Feng;Xie De-Ti
土壤pH值是影响土壤氨氧化菌（AO）生态特性的重要因素之一，AO介导氨通过亚硝酸盐转化为硝酸盐，并对硝酸盐向地下水的淋溶和大气氧化亚氮（N2 O）的产生有重要作用。然而，在酸性紫色土，这是广泛分布在中国西南地区的AO群落的动态在很大程度上仍然是未知的。本研究选取了两种典型的pH值不同的紫色土（酸性：ACI;碱性：ALK）进行研究。采用实时荧光定量PCR技术测定了氨氧化细菌（AOB）和古细菌（AOA）的氨单加氧酶基因amoA和1，5-二磷酸核酮糖羧化酶/加氧酶基因bbL的丰度，并通过克隆和测序分析了AOB和AOA的群落结构。结果表明，ACI紫色土样品中AOB和AOA的丰度均显著低于ALK样品，但AOA/AOB比值高于ALK样品。两种土壤中cbbL基因的丰度差异不显著，但ACI土壤中AOB和AOAamoAtocbbL基因的比例高于ALK土壤。此外，ALK和ACI土壤窝藏AO的对比社区组成。ALK土壤中AOB以3a群占优势（87%），ACI土壤中3a群所占比例下降，9群和10群占77%左右，亚硝化球菌和亚硝化菌分别是ALK和ACI土壤中AOB的主要类群。研究结果揭示了pH、AO和化能自养细菌之间的潜在关系，表明pH可能对AO在紫色土中的适应和选择产生重要影响。
Soil pH has been suggested as one of the most important factors affecting the ecological characteristics of soil ammonia-oxidizers (AO), which mediate the conversion of ammonia to nitrate via nitrite and contribute significantly to the leaching of nitrate to groundwater and the production of atmospheric nitrous oxide (N2O). However, the dynamics of the AO community in acidic purple soils, which are widely distributed in Southwest China, remain largely unknown. In this study, two typical purple soils with different pH values (acidic: ACI; alkaline: ALK) were collected and studied. The abundance ofamoA(gene encoding ammonia monooxygenase) of ammonia-oxidizing bacteria (AOB) and archaea (AOA) and that of thecbbLgene (encoding ribulose-1,5-biphosphate carboxylase/oxygenase) were determined by real-time PCR, and the community structures of AOB and AOA were investigated by cloning and sequencing. The results revealed that abundances of AOB and AOA were significantly lower in the ACI purple soil sample than in the ALK sample, but a higher ratio of AOA to AOB was found in the ACI purple soil sample. No significant difference in the abundance ofcbbLwas found between the two soils, but the ratio of AOB and AOAamoAtocbbLgenes in the ACI soil samples was higher than that in the ALK sample. Moreover, the ALK and ACI soils harbored contrasting community compositions of AO. AOB in the ALK were dominated by cluster 3a (87 %), while the percentage of cluster 3a decreased and clusters 9 and 10 accounted for almost 77 % of the AOB community in the ACI soil.NitrososphaeraandNitrosotaleawere the major AOA phylotypes in the ALK and ACI soils, respectively. In conclusion, our results revealed the potential relations among pH, AO, and total chemoautotrophic bacteria in soil and that pH might have an essential impact on the adaptation and selection of AO in purple soils.
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影响因子：
3.7
作者：
Blainey PC;Mosier AC;Potanina A;Francis CA;Quake SR
通讯作者：
Quake SR
影响因子：
3.6
作者：
Janet Andert;Ella Wessén;G. Börjesson;S. Hallin
通讯作者：
Janet Andert;Ella Wessén;G. Börjesson;S. Hallin
DOI：
10.1073/pnas.1109000108
发表时间：
2011-12-27
影响因子：
11.1
作者：
Gubry-Rangin, Cecile;Hai, Brigitte;Nicol, Graeme W.
通讯作者：
Nicol, Graeme W.
影响因子：
4.8
作者：
Ella Wessén;K. Nyberg;J. Jansson;S. Hallin
通讯作者：
Ella Wessén;K. Nyberg;J. Jansson;S. Hallin
影响因子：
5.1
作者：
Nicol, Graeme W.;Leininger, Sven;Prosser, James I.
通讯作者：
Prosser, James I.