New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.
New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.
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DOI：
10.1038/mtna.2014.34
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发表时间：
2014-08-05
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Single-stranded oligonucleotide aptamers have attracted great attention in the past decade because of their diagnostic and therapeutic potential. These versatile, high affinity and specificity reagents are selected by an iterative in vitro process called SELEX, Systematic Evolution of Ligands by Exponential Enrichment. Numerous SELEX methods have been developed for aptamer selections; some that are simple and straightforward, and some that are specialized and complicated. The method of SELEX is crucial for selection of an aptamer with desired properties; however, success also depends on the starting aptamer library, the target molecule, aptamer enrichment monitoring assays, and finally, the analysis and characterization of selected aptamers. Here, we summarize key recent developments in aptamer selection methods, as well as other aspects of aptamer selection that have significant impact on the outcome. We discuss potential pitfalls and limitations in the selection process with an eye to aid researchers in the choice of a proper SELEX strategy, and we highlight areas where further developments and improvements are desired. We believe carefully designed multiplexed selection methods, when complemented with high-throughput downstream analysis and characterization assays, will yield numerous high-affinity aptamers to protein and small molecule targets, and thereby generate a vast array of reagents for probing basic biological mechanisms and implementing new diagnostic and therapeutic applications in the near future.
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DOI：
10.1007/978-1-59745-557-2_5
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
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作者：
Cerchia, Laura;Giangrande, Paloma H;McNamara, James O;de Franciscis, Vittorio
通讯作者：
de Franciscis, Vittorio
DOI：
10.3390/molecules15010001
发表时间：
2009-12-24
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Avci-Adali M;Paul A;Wilhelm N;Ziemer G;Wendel HP
通讯作者：
Wendel HP
影响因子：
14.8
作者：
Berger, Michael F.;Bulyk, Martha L.
通讯作者：
Bulyk, Martha L.
DOI：
10.1080/15257770802257903
发表时间：
2008-08
期刊：
Nucleosides, nucleotides & nucleic acids
影响因子：
--
作者：
Boese BJ;Corbino K;Breaker RR
通讯作者：
Breaker RR
影响因子：
3.7
作者：
Ahmad KM;Oh SS;Kim S;McClellen FM;Xiao Y;Soh HT
通讯作者：
Soh HT