Cell sorting and germ layer formation in reconstructed starfish embryos
Cell sorting and germ layer formation in reconstructed starfish embryos
复制标题
重建海星胚胎中的细胞分选和胚层形成
DOI：
10.1111/dgd.12749
复制
发表时间：
2021
期刊：
影响因子：
4.6
通讯作者：
H. Kaneko
中科院分区：
文献类型：
--
作者：
Sohei Suzuki;Ikuko Omori;R. Kuraishi;H. Kaneko
胚层的形成是由早期发育阶段的胚胎细胞分选驱动的。海星（Patiria pectinifera）胚胎具有相连的内胚层和外胚层，尽管上皮之间的接触面很少。为了更好地了解细胞分选和胚层形成之间的关联，我们重建了 P. pectinifera 胚胎并检查了它们的胚层形成。初步观察表明，囊胚晚期胚胎体的推定内胚层 (pEN) 和推定外胚层 (pEC) 部分在整个发育过程中得到保留。基于此，从每个真皮碎片分离的细胞在重建实验中混合。我们的结果表明，pEN 和 pEC 细胞分别位于重新聚集体的内部和外部，形成包含两个上皮层的胚胎体，由囊胚腔分隔。在此过程中，pEN 细胞具有活动性，从小细胞团转变为大细胞团。相反，在单独培养物中形成的重新聚集体中，pEN细胞表现出很强的粘附能力，而pEC细胞则经历上皮化。与 pEN 细胞不同，pEC 细胞的重新聚集先于钙粘蛋白表达。在两种重新聚集体中类似地观察到丝状肌动蛋白。这些结果表明，在海星胚胎的重建过程中，胚层的形成是通过 pEN 和 pEC 细胞的分类而发生的，具体取决于它们的粘附性、运动性和上皮化。在体内，这些特性可能体现了构成上皮单层的胚层中细胞粘附的生理意义。
Germ layer formation is driven by embryonic cell sorting during the early developmental stages. Starfish (Patiria pectinifera) embryos have a connected endoderm and ectoderm, albeit with few contact surfaces between the epithelia. To better understand the association between cell sorting and germ layer formation, we reconstructed P. pectinifera embryos and examined their germ layer formation. Initial observations showed that the presumptive endodermal (pEN) and presumptive ectodermal (pEC) portions of the embryonic body at the late‐blastula stage were preserved throughout development. Based on this, cells that were dissociated from each dermal fragment were mixed in a reconstruction experiment. Our results showed that the pEN and pEC cells were located inside and outside the reaggregates, respectively, to form an embryonic body containing two epithelial layers, separated by a blastocoel. During this process, the pEN cells were motile and shifted from smaller clumps to form a large clump. In contrast, in reaggregates formed in separate cultures, the pEN cells showed strong adhesion abilities, whereas the pEC cells underwent epithelialization. Unlike that in pEN cells, the reaggregation of pEC cells preceded cadherin expression. Filamentous actin was similarly observed in both reaggregates. These results suggest that during the reconstruction of starfish embryos, germ layer formation occurs via the sorting of pEN and pEC cells, depending on their adhesiveness, motility, and epithelialization. In vivo, these properties might embody the physiological significance of cell adhesion in the germ layers constituting the epithelial monolayer.
影响因子：
2.7
作者：
Duguay, D;Foty, RA;Steinberg, MS
通讯作者：
Steinberg, MS
影响因子：
2.7
作者：
Foty, RA;Steinberg, MS
通讯作者：
Steinberg, MS