Nuclear shift of hnRNP K protein in neoplasms and other states of enhanced cell proliferation.
Nuclear shift of hnRNP K protein in neoplasms and other states of enhanced cell proliferation.
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DOI：
10.1038/sj.bjc.6601250
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发表时间：
2003-10-20
影响因子：
8.8
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中科院分区：
文献类型：
--
作者：
异质核核糖核蛋白 K (hnRNP K) 是一种普遍表达的蛋白质，与信号转导器、调节基因表达以及选择性 RNA 和 DNA 基序的蛋白质相互作用。 K 蛋白响应细胞外信号而被修饰，并直接调节转录和翻译速率。我们使用血清处理的肝细胞培养物、部分肝切除术后的肝脏和肝肿瘤作为系统来比较 K 蛋白在静止和分裂细胞中的表达、亚细胞分布和酪氨酸磷酸化。结果显示，在增殖增强的状态下，K蛋白mRNA的表达增加。与静息细胞相比，增殖时核 K 蛋白的水平也更高。相反，与静止细胞相比，分裂时细胞质 K 蛋白的水平相同或更低。增殖增强的状态也与 K 蛋白酪氨酸磷酸化水平的增加有关。分裂细胞中 K 蛋白的核移位可能反映了 K 蛋白参与调节增殖细胞中基因表达的多个信号事件。
The heterogeneous nuclear ribonucleoprotein K (hnRNP K), is a ubiquitously expressed protein that interacts with signal transducers, proteins that modulate gene expression and selective RNA and DNA motifs. K protein is modified in response to extracellular signals and directly regulates rates of transcription and translation. We used serum-treated hepatocyte culture, liver after partial hepatectomy and hepatic neoplasms as systems to compare expression, subcellular distribution and tyrosine phosphorylation of K protein in quiescent and dividing cells. The results show that expression of K protein mRNA was increased in states of enhanced proliferation. Levels of nuclear K protein were also higher in proliferating compared to resting cells. In contrast, levels of cytoplasmic K protein were the same or lower in dividing compared to quiescent cells. States of enhanced proliferation were also associated with increased levels of K protein tyrosine phosphorylation. Nuclear shift of K protein in dividing cells may reflect involvement of K protein in signalling multiple events that regulate expression of genes in proliferating cells.
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