Mg-Protoporphyrin IX Signals Enhance Plant's Tolerance to Cold Stress.
Mg-Protoporphyrin IX Signals Enhance Plant's Tolerance to Cold Stress.
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DOI：
10.3389/fpls.2016.01545
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发表时间：
2016
影响因子：
5.6
通讯作者：
Yuan S
中科院分区：
文献类型：
--
作者：
Zhang ZW;Wu ZL;Feng LY;Dong LH;Song AJ;Yuan M;Chen YE;Zeng J;Chen GD;Yuan S
研究了Mg-原卟啉IX（Mg-Proto IX）信号与植物耐冷性的关系。将生长3周的拟南芥幼苗在室温下用2 mM谷氨酸（Glu）和2 mM MgCl 2预处理48小时以诱导Mg-Proto IX积累。然后在4°C下再进行冷应激72小时。Glu + MgCl 2预处理通过诱导Mg-Proto IX信号，提高叶片温度，显著缓解了随后的低温胁迫。Glu + MgCl 2处理的保护作用在Mg-Proto IX合成、Mg-Proto IX信号传导和抗氰化物呼吸的突变体中大大受损。Mg-Proto IX合成突变体、Mg-Proto IX信号突变体和阿坝信号突变体的低温响应基因表达增强受到显著影响，而抗氰呼吸突变体的低温响应基因表达增强不受影响。低温胁迫显著促进了叶片的抗氰呼吸和总呼吸，Glu + MgCl 2处理可进一步促进抗氰呼吸和总呼吸。Mg-Proto IX信号还激活抗氧化酶并增加非酶抗氧化剂[谷胱甘肽而不是抗坏血酸（阿萨）]以在冷应激期间维持氧化还原平衡。
The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate (Glu) and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4°C for additional 72 h. Glu + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of Glu + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling, and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the Glu + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants [glutathione but not ascorbic acid (AsA)] to maintain redox equilibrium during the cold stress.
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