Detection of the circulating antigen 14-3-3 protein of Schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits.
Detection of the circulating antigen 14-3-3 protein of Schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits.
复制标题
时间分辨荧光免疫法检测兔日本血吸虫循环抗原14-3-3蛋白
DOI：
10.1186/1756-3305-4-95
复制
发表时间：
2011-05-28
影响因子：
3.2
通讯作者：
Ke XD
中科院分区：
文献类型：
--
作者：
Qian CY;Huang B;Yu CX;Zhang J;Yin XR;Wang J;Song LJ;Zhang W;Ke XD
背景
血吸虫病仍然是一个主要的公共卫生问题，困扰着全世界数百万人。低水平的血吸虫感染需要更敏感的诊断方法。本研究建立了检测日本血吸虫循环抗原信号转导蛋白14-3-3的时间分辨荧光免疫分析法（TRFIA）。
结果
14-3-3-TRFIA的检出限为0.78 ng/ml，线性范围为0.78 ~ 800 ng/ml。该方法的批内和批间变异性分别为8.9%和12.2%，平均回收率为92.1%~ 115.5%。在感染后的前21天内，14-3-3-TRFIA的阳性率明显高于ELISA。说明14-3-3-TRFIA对血吸虫活动性感染有较高的检测效率，是一种较好的早期诊断方法。
结论
建立了检测沙门氏菌循环抗原14-3-3的夹心TRFIA。日本血吸虫病的诊断方法已被证明是一种很好的潜在的诊断方法。
Background
Schistosomiasis remains a major public health concern that afflicts millions of people worldwide. Low levels of Schistosoma infection require more sensitive diagnostic methods. In this study, a time-resolved fluoroimmunoassay (TRFIA) was developed for detecting the signal transduction protein 14-3-3, a circulating antigen of Schistosoma japonicum.
Results
The detection limit of 14-3-3-TRFIA was 0.78 ng/ml, with a linear measurement range from 0.78 to 800 ng/ml. The average intra-assay and inter-assay variability of this TRFIA was 8.9% and 12.2% respectively, and the mean recovery rate ranged from 92.1% to 115.5%. Within the first 21 days post-infection in rabbits, the positive rates of the 14-3-3-TRFIA were distinctly higher compared to ELISA. All these findings illustrate that 14-3-3-TRFIA has a higher detection efficacy and is a good early diagnostic method for active Schistosoma infection.
Conclusions
A sandwich TRFIA for detecting the circulating antigen 14-3-3 of S. japonicum has been developed, and has demonstrated to be a good potential diagnostic method for schistosomiasis.
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DOI：
10.1016/s0732-8893(00)00207-8
发表时间：
2000-12-01
影响因子：
2.9
作者：
Zhu, CL;Tian, HS;Guan, XH
通讯作者：
Guan, XH
影响因子：
6.2
作者：
Huang, B;Xiao, HL;Jin, J
通讯作者：
Jin, J
影响因子：
6.1
作者：
Li Shi-zhu;Luz, Acosta;Zhou Xiao-nong
通讯作者：
Zhou Xiao-nong
影响因子：
2.7
作者：
Andoh, T;Nagasawa, H
通讯作者：
Nagasawa, H
一项为期5年的纵向研究，对中华民国四川省安宁河谷的Shian Village的血吸虫病传播。
影响因子：
3.2
作者：
Zhu R;Gray DJ;Thrift AP;Williams GM;Zhang Y;Qiu DC;Zheng F;Li YS;Guo J;Zhu HQ;Wu WP;Li RS;McManus DP
通讯作者：
McManus DP