Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum.
Microfluidic electrochemical immunoarray for ultrasensitive detection of two cancer biomarker proteins in serum.
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DOI：
10.1016/j.bios.2011.05.005
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发表时间：
2011-07-15
影响因子：
12.6
通讯作者：
Rusling, James F.
中科院分区：
文献类型：
--
作者：
Chikkaveeraiah, Bhaskara V.;Mani, Vigneshwaran;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
关键词：
采用模制聚二甲基硅氧烷通道和常规机加工部件与泵和样品进样器接口，制备了用于癌症蛋白生物标志物多路检测的微流控电化学免疫分析系统。使用高酶标记的1 μm超顺磁颗粒（MP）抗体偶联物离线捕获分析物，并捕获抗体附着在8电极测量芯片上，同时检测血清中前列腺特异性抗原（PSA）和白细胞介素-6 （IL-6）的亚pg mL−1水平。MPs与~90,000抗体和~200,000辣根过氧化物酶（HRP）标签结合，提供高效的离线捕获和高灵敏度。测量电极具有一层5nm的谷胱甘肽修饰的金纳米颗粒，以附着捕获mp分析物生物偶联物的抗体。在稀释的血清混合物中，PSA的检出限为0.23 pg mL - 1， IL-6的检出限为0.30 pg mL - 1。在总检测时间1.15 h内检测前列腺癌患者血清中PSA和IL-6生物标志物，传感器阵列结果与标准酶联免疫吸附试验（ELISA）具有良好的相关性。这些微流控免疫传感器采用纳米结构表面和离线分析物捕获与重标记的顺磁颗粒，对诊断癌症生物标志物的准确，敏感的多路检测具有很大的希望。
A microfluidic electrochemical immunoassay system for multiplexed detection of protein cancer biomarkers was fabricated using a molded polydimethylsiloxane channel and routine machined parts interfaced with a pump and sample injector. Using off-line capture of analytes by heavily-enzyme-labeled 1 μm superparamagnetic particle (MP)-antibody bioconjugates and capture antibodies attached to an 8-electrode measuring chip, simultaneous detection of cancer biomarker proteins prostate specific antigen (PSA) and interleukin-6 (IL-6) in serum was achieved at sub-pg mL−1 levels. MPs were conjugated with ~90,000 antibodies and ~200,000 horseradish peroxidase (HRP) labels to provide efficient off-line capture and high sensitivity. Measuring electrodes feature a layer of 5 nm glutathione-decorated gold nanoparticles to attach antibodies that capture MP-analyte bioconjugates. Detection limits of 0.23 pg mL−1 for PSA and 0.30 pg mL−1 for IL-6 were obtained in diluted serum mixtures. PSA and IL-6 biomarkers were measured in serum of prostate cancer patients in total assay time 1.15 h and sensor array results gave excellent correlation with standard enzyme-linked immunosorbent assays (ELISA). These microfluidic immunosensors employing nanostructured surfaces and off-line analyte capture with heavily-labeled paramagnetic particles hold great promise for accurate, sensitive multiplexed detection of diagnostic cancer biomarkers.
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影响因子：
12.6
作者：
Ghindilis, AL;Atanasov, P;Wilkins, E
通讯作者：
Wilkins, E
影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
5.4
作者：
Chikkaveeraiah BV;Liu H;Mani V;Papadimitrakopoulos F;Rusling JF
通讯作者：
Rusling JF
影响因子：
7.4
作者：
Hermann, Marc;Veres, Teodor;Tabrizian, Maryam
通讯作者：
Tabrizian, Maryam
影响因子：
16.6
作者：
Krishnan, Sadagopan;Mani, Vigneshwaran;Wasalathanthri, Dhanuka;Kumar, Challa V.;Rusling, James F.
通讯作者：
Rusling, James F.