Quorum sensing in bacterial species that use degenerate autoinducers can be tuned by using structurally identical non-native ligands.
Quorum sensing in bacterial species that use degenerate autoinducers can be tuned by using structurally identical non-native ligands.
复制标题
DOI：
10.1002/cbic.201000551
复制
发表时间：
2011-01-03
期刊：
影响因子：
3.2
通讯作者：
Blackwell, Helen E.
中科院分区：
文献类型：
--
作者：
Palmer, Andrew G.;Streng, Evan;Jewell, Kelsea A.;Blackwell, Helen E.
许多细菌使用群体感应（QS）来调节细胞密度依赖性表型，这些表型在维持它们与真核宿主的关联中起着关键作用。在革兰氏阴性菌中，QS主要由n -酰基化l-同型丝氨酸内酯（AHL）信号及其同源luxr型受体控制。ahl - luxr型受体结合调节QS表型所必需的靶基因的表达。我们最近发现了一系列能够阻断海洋共生费氏弧菌中AHL-LuxR结合的非天然ahl，从而在该生物中强烈促进或抑制QS。V. fischeri以N-(3-oxo)-hexanoyl - hl （OHHL）作为其主要的QS信号，OHHL也被其他几种细菌用作QS信号。这种信号退化在细菌中很常见，我们试图确定在费氏弧菌中活跃的非天然LuxR激动剂和拮抗剂是否也会调节其他使用OHHL的细菌的QS表型。在此，我们报道了一组合成LuxR调节剂在植物病原体胡萝卜酸乳杆菌亚种中的活性研究。胡萝卜Ecc71。该病原体使用OHHL和两个密切相关的luxr型受体ExpR1和ExpR2来控制毒力，我们通过量化毒力因子的产生来评估它们对合成配体的反应。我们的研究结果表明，P. carotovora Ecc71和V. fischeri的ExpR受体之间的配体活性趋势总体上保持不变，并表明这些化合物可以作为研究细菌QS的工具。然而，某些化合物在活性上的显著差异是明显的，这表明可能有选择性地调节细菌中利用退化ahl的QS。
Many bacteria use quorum sensing (QS) to regulate cell-density dependent phenotypes that play critical roles in the maintenance of their associations with eukaryotic hosts. In Gram-negative bacteria, QS is primarily controlled by N-acylated l-homoserine lactone (AHL) signals and their cognate LuxR-type receptors. AHL-LuxR-type receptor binding regulates the expression of target genes necessary for QS phenotypes. We recently identified a series of non-native AHLs capable of intercepting AHL-LuxR binding in the marine symbiont Vibrio fischeri, and thereby strongly promoting or inhibiting QS in this organism. V. fischeri utilizes N-(3-oxo)-hexanoyl l-HL (OHHL) as its primary QS signal, and OHHL is also used by several other bacterial species for QS. Such signal degeneracy is common among bacteria, and we sought to determine if our non-native LuxR agonists and antagonists, which are active in V. fischeri, would also modulate QS phenotypes in other bacteria that use OHHL. Herein, we report investigations into the activity of a set of synthetic LuxR modulators in the plant pathogen Pectobacterium carotovora subsp. carotovora Ecc71. This pathogen uses OHHL and two closely related LuxR-type receptors, ExpR1 and ExpR2, to control virulence, and we evaluated their responses to synthetic ligands by quantifying virulence factor production. Our results suggest an overall conservation in the activity trends of the ligands between the ExpR receptors in P. carotovora Ecc71 and LuxR in V. fischeri, and indicate that these compounds could be used as tools to study QS in an expanded set of bacteria. Notable differences in activity were apparent for certain compounds, however, and suggest that it might be possible to selectively regulate QS in bacteria that utilize degenerate AHLs.
登录
查看更多内容
影响因子：
3.2
作者：
Glansdorp, FG;Thomas, GL;Spring, DR
通讯作者：
Spring, DR
影响因子：
3.2
作者：
Gurich, Nataliya;Gonzalez, Juan E.
通讯作者：
Gonzalez, Juan E.
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
15
作者：
Geske, Grant D.;O'Neill, Jennifer C.;Blackwell, Helen E.
通讯作者：
Blackwell, Helen E.
影响因子：
3.2
作者：
EBERHARD, A
通讯作者：
EBERHARD, A