The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis.
The release of microparticles by Jurkat leukemia T cells treated with staurosporine and related kinase inhibitors to induce apoptosis.
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DOI：
10.1007/s10495-010-0470-3
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发表时间：
2010-05
期刊：
影响因子：
7.2
通讯作者：
Pisetsky, David S.
中科院分区：
文献类型：
--
作者：
Ullal, Anirudh J.;Pisetsky, David S.
微粒（MP）是从经历活化或细胞死亡的细胞释放的小的膜结合囊泡。这些颗粒显示出潜在的生物活性，可以影响生理和病理过程。先前对Jurkat T白血病细胞系的研究表明，星形孢菌素（STS）在细胞经历凋亡时诱导MP的释放。为了进一步研究这一过程，我们测试了STS，其类似物，7-hydroxystaurosporine（UCN-01），和其他蛋白激酶C（PKC）和细胞周期蛋白依赖性激酶（CDK）抑制剂的影响。使用FACS分析来评估MP释放。这些研究的结果表明，STS和UCN-01诱导Jurkat细胞MP释放;相反，其他PKC和CDK抑制剂未能诱导相当的释放，这表明释放不是由单独抑制任一激酶引起的。时程实验表明，STS诱导的颗粒释放发生早在治疗后2小时，与早期释放MP显示低水平的结合膜联蛋白V和碘化丙啶（PI）。然而，早期释放的MP在培养中成熟为膜联蛋白V和PI阳性表型。总之，这些结果表明STS和UCN-01诱导表型不同的MP，并反映了细胞凋亡期间激酶抑制的特定模式。
Microparticles (MPs) are small membrane-bound vesicles released from cells undergoing activation or cell death. These particles display potent biological activities that can impact on physiologic and pathologic processes. Previous studies with the Jurkat T leukemia cell line demonstrated that staurosporine (STS) induces the release of MPs as cells undergo apoptosis. To investigate further this process, we tested the effects of STS, its analogue, 7-hydroxystaurosporine (UCN-01), and other protein kinase C (PKC) and cyclin-dependent kinase (CDK) inhibitors. FACS analysis was used to assess MP release. Results of these studies indicate that STS and UCN-01 induce MP release by Jurkat cells; in contrast, other PKC and CDK inhibitors failed to induce comparable release, suggesting that release does not result from simple inhibition of either kinase alone. Time course experiments indicated that STS-induced particle release occurred as early as 2 h after treatment, with the early release MPs displaying low levels of binding of annexin V and propidium iodide (PI). Early-release MPs, however, matured in culture to an annexin V- and PI-positive phenotype. Together, these results indicate that STS and UCN-01 induce MPs that are phenotypically distinct and reflect specific patterns of kinase inhibition during apoptosis.
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影响因子：
7.2
作者：
Distler, JHW;Huber, LC;Pisetsky, DS
通讯作者：
Pisetsky, DS
影响因子：
6.7
作者：
Hron, Gregor;Kollars, Marietta;Weltermann, Ansgar
通讯作者：
Weltermann, Ansgar
DOI：
10.1161/01.atv.0000249639.36915.04
发表时间：
2006-12-01
影响因子：
8.7
作者：
Chironi, Gilles;Simon, Alain;Tedgui, Alain
通讯作者：
Tedgui, Alain
影响因子：
6.4
作者：
Janowska-Wieczorek, A;Wysoczynski, M;Ratajczak, MZ
通讯作者：
Ratajczak, MZ
影响因子：
3.7
作者：
Hunter, Melissa Piper;Ismail, Noura;Zhang, Xiaoli;Aguda, Baltazar D.;Lee, Eun Joo;Yu, Lianbo;Xiao, Tao;Schafer, Jeffrey;Lee, Mei-Ling Ting;Schmittgen, Thomas D.;Nana-Sinkam, S. Patrick;Jarjoura, David;Marsh, Clay B.
通讯作者：
Marsh, Clay B.