Fat Body Cells Are Motile and Actively Migrate to Wounds to Drive Repair and Prevent Infection.
Fat Body Cells Are Motile and Actively Migrate to Wounds to Drive Repair and Prevent Infection.
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脂肪体细胞是运动的，并积极迁移到伤口以驱动修复并防止感染。
DOI：
10.1016/j.devcel.2018.01.026
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发表时间：
2018-02-26
影响因子：
11.8
通讯作者：
Martin P
中科院分区：
文献类型：
--
作者：
Franz A;Wood W;Martin P
脂肪细胞在各种组织中具有除能量储存之外的许多功能，包括调节代谢、生长和免疫。然而，人们对它们在伤口愈合中的作用知之甚少。在这里，我们使用脂肪体细胞，相当于果蝇中的脊椎动物脂肪细胞的活体成像，来研究它们在皮肤损伤后的潜在行为和功能。我们发现，蛹脂肪体细胞是不动的，如前所述，但积极迁移到伤口使用一个不寻常的粘附独立，肌动球蛋白驱动，蠕动模式的运动。一旦在伤口处，脂肪体细胞与血细胞、果蝇巨噬细胞合作，以清除伤口的细胞碎片;它们还紧密密封上皮伤口间隙并局部释放抗菌肽以对抗伤口感染。因此，脂肪体细胞是运动细胞，使它们能够迁移到伤口，以承担驱动伤口修复和预防感染所需的几种局部功能。脂肪体细胞通过在伤口边缘周围形成片状伪足而紧密地密封差距。脂肪体细胞与巨噬细胞协作以清除伤口碎片。脂肪体细胞在感染的伤口局部释放抗微生物肽。现在显示，后者可以使用类似于南极洲的“游泳”运动性主动迁移到伤口。一旦到达那里，它们就多任务处理以清除伤口细胞碎片，堵塞上皮间隙，并上调AMP以防止感染。
Adipocytes have many functions in various tissues beyond energy storage, including regulating metabolism, growth, and immunity. However, little is known about their role in wound healing. Here we use live imaging of fat body cells, the equivalent of vertebrate adipocytes in Drosophila, to investigate their potential behaviors and functions following skin wounding. We find that pupal fat body cells are not immotile, as previously presumed, but actively migrate to wounds using an unusual adhesion-independent, actomyosin-driven, peristaltic mode of motility. Once at the wound, fat body cells collaborate with hemocytes, Drosophila macrophages, to clear the wound of cell debris; they also tightly seal the epithelial wound gap and locally release antimicrobial peptides to fight wound infection. Thus, fat body cells are motile cells, enabling them to migrate to wounds to undertake several local functions needed to drive wound repair and prevent infections. Fat body cells actively migrate to wounds using a peristaltic mode of motility Fat body cells tightly seal the gap by forming lamellipodia around the wound margin Fat body cells collaborate with macrophages to clear wound debris Fat body cells locally release antimicrobial peptides at infected wounds Adipocytes and their fly equivalent, fat body cells, have been considered immotile, but Franz et al. now show the latter can actively migrate to wounds using a peristaltic-like “swimming” motility. Once there, they multitask to clear wound cell debris, plug the epithelial gap, and upregulate AMPs to prevent infection.
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影响因子：
9.8
作者：
Grönke S;Müller G;Hirsch J;Fellert S;Andreou A;Haase T;Jäckle H;Kühnlein RP
通讯作者：
Kühnlein RP
DOI：
10.1083/jcb.201211039
发表时间：
2013-07-22
期刊：
The Journal of cell biology
影响因子：
--
作者：
Antunes M;Pereira T;Cordeiro JV;Almeida L;Jacinto A
通讯作者：
Jacinto A
影响因子：
4.6
作者：
Gates, Julie;Mahaffey, James P.;Peifer, Mark
通讯作者：
Peifer, Mark
影响因子：
4.6
作者：
Cherbas, L;Hu, X;Cherbas, P
通讯作者：
Cherbas, P
影响因子：
56.9
作者：
Delanoue, Renald;Meschi, Eleonora;Leopold, Pierre
通讯作者：
Leopold, Pierre