Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding.
Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding.
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DOI：
10.1083/jcb.201211039
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发表时间：
2013-07-22
期刊：
影响因子：
--
通讯作者：
Jacinto A
中科院分区：
文献类型：
--
作者：
Antunes M;Pereira T;Cordeiro JV;Almeida L;Jacinto A
上皮损伤引起肌动球蛋白流波和顶端细胞收缩，这取决于钙信号和肌动蛋白丝切断。上皮伤口愈合依赖于组织运动和细胞形状变化。我们的工作表明，创伤后，立即有一个戏剧性的细胞骨架重塑，包括一个脉冲的肌动球蛋白细丝，组装在伤口边缘周围的细胞，并从细胞流向伤口边缘的细胞。我们表明，这肌动球蛋白流调节的透明和岩石，它引起了一波顶端细胞收缩，最终形成的前沿肌动球蛋白电缆，结构是必不可少的伤口闭合。钙信号在这一过程中发挥了重要作用，因为它的细胞内浓度急剧增加后立即受伤，和下调瞬时受体电位通道M，应激激活的钙通道，也损害了肌动球蛋白流。降低凝溶胶蛋白（一种已知的钙激活肌动蛋白抑制性切断蛋白）的活性也会损害伤口反应，这表明切割现有的肌动蛋白丝网络是细胞骨架重塑过程的重要组成部分。
Epithelial wounding causes waves of actomyosin flow and apical cell constriction that are dependent on calcium signaling and actin filament severing. Epithelial wound healing relies on tissue movements and cell shape changes. Our work shows that, immediately after wounding, there was a dramatic cytoskeleton remodeling consisting of a pulse of actomyosin filaments that assembled in cells around the wound edge and flowed from cell to cell toward the margin of the wound. We show that this actomyosin flow was regulated by Diaphanous and ROCK and that it elicited a wave of apical cell constriction that culminated in the formation of the leading edge actomyosin cable, a structure that is essential for wound closure. Calcium signaling played an important role in this process, as its intracellular concentration increased dramatically immediately after wounding, and down-regulation of transient receptor potential channel M, a stress-activated calcium channel, also impaired the actomyosin flow. Lowering the activity of Gelsolin, a known calcium-activated actin filament–severing protein, also impaired the wound response, indicating that cleaving the existing actin filament network is an important part of the cytoskeleton remodeling process.
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