Capillary Network-Like Organization of Endothelial Cells in PEGDA Scaffolds Encoded with Angiogenic Signals via Triple Helical Hybridization.
Capillary Network-Like Organization of Endothelial Cells in PEGDA Scaffolds Encoded with Angiogenic Signals via Triple Helical Hybridization.
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DOI：
10.1002/adfm.201303217
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发表时间：
2014-06-04
影响因子：
19
通讯作者：
Yu, S. M.
中科院分区：
文献类型：
--
作者：
Stahl, Patrick J.;Chan, Tania R.;Shen, Yu-I;Sun, Guoming;Gerecht, Sharon;Yu, S. M.
组织工程构建体在植入后的存活取决于适当的血管化。内皮细胞分化为成熟的微血管需要细胞、支架和生长因子之间的动态相互作用，这在人工系统中难以重现。以前，报道了显示胶原模拟肽（CMP）的光交联聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶，称为PEGDA-CMP，其可以通过CMP-CMP三螺旋杂交与生物活性分子进一步缀合。在此，显示了具有模拟血管内皮生长因子（VEGF）的促血管生成结构域和可折叠成三螺旋构象的胶原模拟结构域的双功能肽可与PEGDA-CMP水凝胶的CMP侧链杂交，这导致向内皮细胞呈递不溶性VEGF样信号。以这种方式在微图案化支架表面上呈现VEGF样信号将细胞从静止状态转变为伸长和对齐的表型，表明该系统可用于工程化组织化微血管。研究还表明，促血管生成肽与改性葡聚糖/PEGDA水凝胶联合局部应用时，可增强小鼠烧伤伤口的新血管形成，证明CMP介导的基质结合生物活性分子在皮肤损伤中的潜在临床用途。
Survival of tissue engineered constructs after implantation depends on proper vascularization. The differentiation of endothelial cells into mature microvasculature requires dynamic interactions between cells, scaffold, and growth factors, which are difficult to recapitulate in artificial systems. Previously, photocrosslinked poly(ethylene glycol) diacrylate (PEGDA) hydrogels displaying collagen mimetic peptides (CMPs), dubbed PEGDA‐CMP, that can be further conjugated with bioactive molecules via CMP‐CMP triple helix hybridization were reported. Here, it is shown that a bifunctional peptide featuring pro‐angiogenic domain mimicking vascular endothelial growth factor (VEGF) and a collagen mimetic domain that can fold into a triple helix conformation can hybridize with CMP side chains of the PEGDA‐CMP hydrogel, which results in presentation of insoluble VEGF‐like signals to endothelial cells. Presentation of VEGF‐like signals on the surface of micropatterned scaffolds in this way transforms cells from a quiescent state to elongated and aligned phenotype suggesting that this system could be used to engineer organized microvasculature. It is also shown that the pro‐angiogenic peptide, when applied topically in combination with modified dextran/PEGDA hydrogels, can enhance neovascularization of burn wounds in mice demonstrating the potential clinical use of CMP‐mediated matrix‐bound bioactive molecules for dermal injuries.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1073/pnas.0505047102
发表时间：
2005-10-04
影响因子：
11.1
作者：
D'Andrea, LD;Iaccarino, G;Pedone, C
通讯作者：
Pedone, C
影响因子：
3.4
作者：
Dickinson, Laura E.;Moura, Matthew E.;Gerecht, Sharon
通讯作者：
Gerecht, Sharon
影响因子：
14.8
作者：
Brafman, David A.;Chien, Shu;Willert, Karl
通讯作者：
Willert, Karl
影响因子：
4.8
作者：
Ackerman, MS;Bhate, M;Brodsky, B
通讯作者：
Brodsky, B