Serotype‐specific antigen ELISA for detection of Chiba virus in stools
Serotype‐specific antigen ELISA for detection of Chiba virus in stools
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血清型特异性抗原 ELISA 检测粪便中的千叶病毒
DOI：
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发表时间：
2000
影响因子：
12.7
通讯作者：
Yasumoto Suzuki
中科院分区：
文献类型：
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作者：
Shinichi Kobayashi;K. Sakae;K. Natori;N. Takeda;T. Miyamura;Yasumoto Suzuki
千叶病毒（CV）是一种诺瓦克样病毒（NLV），于1987年首次被确定为日本千叶县发生的牡蛎相关胃肠炎暴发的原因。开发了一种基于重组杆状病毒表达的CV衣壳蛋白（rCV）的超免疫抗血清的酶联免疫吸附试验（ELISA），用于检测粪便中的CV抗原。未观察到与其他肠道病毒（包括肠道病毒、轮状病毒、星状病毒或肠道腺病毒）的交叉反应。ELISA用于筛选从16起牡蛎相关急性胃肠炎爆发中收集的101份粪便。7次暴发的12份粪便（11.9%）为CV抗原阳性。通过RT-PCR和核苷酸测序确认了10株rCV ELISA阳性菌株。ELISA阳性菌株彼此显示96-100%的核苷酸序列同一性，尽管它们相隔9年获得。系统发育分析表明，所有10株病毒与基因组I病毒的原型CV聚类。我们得出结论，本研究中描述的抗原ELISA具有高度的型特异性，该方法可用于千叶病毒感染的流行病学调查。J. Med. Virol. 62：233-238，2000.© 2000 Wiley‐利斯公司
Chiba virus (CV), a Norwalk‐like virus (NLV), was first identified as a cause of oyster‐associated outbreak of gastroenteritis that occurred in Chiba prefecture, Japan, in 1987. An enzyme‐linked immunosorbent assay (ELISA), based on hyperimmune antisera to recombinant baculovirus‐expressed capsid proteins of CV (rCV), was developed to detect CV antigen in stools. No cross‐reactions were observed with other enteric viruses including enteroviruses, rotaviruses, astroviruses, or enteric adenoviruses. The ELISA was used to screen 101 stools collected from 16 oyster‐associated outbreaks of acute gastroenteritis. Twelve stools (11.9%) from seven outbreaks were positive for CV antigen. Ten rCV ELISA‐positive strains were confirmed by RT‐PCR and nucleotide sequencing. ELISA‐positive strains showed 96–100% nucleotide sequence identity to each other, though they were obtained nine years apart. Phylogenetic analysis demonstrated that all ten strains clustered with the prototype CV in genogroup I viruses. We concluded that the antigen ELISA described in this study is highly type‐specific, and that this method should be useful for epidemiological surveys of Chiba virus infections. J. Med. Virol. 62:233–238, 2000. © 2000 Wiley‐Liss, Inc.
影响因子：
3.7
作者：
JIANG, X;WANG, M;ESTES, MK
通讯作者：
ESTES, MK