Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome.
Changes of cytokine levels in a mouse model of post-infectious irritable bowel syndrome.
复制标题
感染后肠易激综合征小鼠模型细胞因子水平的变化
DOI：
10.1186/s12876-015-0272-8
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发表时间：
2015-04-01
影响因子：
2.4
通讯作者：
Lan C
中科院分区：
文献类型：
--
作者：
Yang B;Zhou X;Lan C
背景肠易激综合征（Irritable bowel syndrome，IBS）是一种高度流行的功能性胃肠道疾病.感染后IBS（PI-IBS）是由症状发作前的急性胃肠道感染引起的。然而，PI-IBS的病理生理学尚不清楚，本研究的目的是探讨可能的免疫机制PI-IBS。MethodsC 57 BL/6小鼠被随机分配到无论是感染组或对照组。感染组用旋毛虫感染小鼠，建立PI-IBS模型（500个旋毛虫），对照组仅给予生理盐水。通过腹部退缩反射评分评价小鼠对结直肠扩张的内脏敏感性，通过苏木精-伊红染色评价肠道炎症;在感染后第56天，采用逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附试验（ELISA）检测肠段特异性细胞因子的mRNA和蛋白水平。与对照组相比，IBS组小鼠十二指肠和回肠中IL-10含量明显增加，空肠、回肠和结肠中IL-10含量下降。IL-1β的表达水平在两组间无显著性差异。结论本研究表明，PI-IBS的病理生理学中的重要组成部分--局部低度炎症和免疫激活主要由特异性细胞因子诱导和维持。
BackgroundIrritable bowel syndrome (IBS) is a highly prevalent functional gastrointestinal disorder. Post-infectious IBS (PI-IBS) is caused by an acute gastrointestinal infection preceding the onset of symptoms. However, the pathophysiology of PI-IBS is not clear, and the purpose of this study was to investigate the probable immune mechanisms of PI-IBS.MethodsC57BL/6 mice were randomly assigned to either an infection group or a control group. Mice in the infection group were infected withTrichinella spiralisto establish a model of PI-IBS (500 Trichinella), while control mice received only salt solution. Visceral sensitivity of colorectal distention in mice was evaluated by abdominal withdrawal reflex scores and intestinal inflammation was assessed using hematoxylin-eosin staining; at day 56 post-infection, the mRNA and protein levels of specific cytokines in the gut segments were detected using reverse-transcription polymerase chain reaction and enzyme-linked immunoabsorbent assay.ResultsLevels of interferon γ and interleukin (IL)-17 in the PI-IBS group were significantly increased in the duodenum and ileum, and IL-10 was decreased in the jejunum, ileum, and colon compared with control mice. However, the expression level of IL-1β was not significantly different between the two groups.ConclusionsThe present study suggests that the local low-grade inflammation and immune activation that are an important component of the pathophysiology of PI-IBS are primarily induced and maintained by specific cytokines.
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影响因子：
15.3
作者：
D'Andrea, A;Aste-Amezaga, M;Valiante, N M;Ma, X;Kubin, M;Trinchieri, G
通讯作者：
Trinchieri, G
影响因子：
7.6
作者：
Corinaldesi, R.;Stanghellini, V.;Barbara, G.
通讯作者：
Barbara, G.
影响因子：
29.4
作者：
Buhner, Sabine;Li, Qin;Schemann, Michael
通讯作者：
Schemann, Michael
影响因子：
6
作者：
Al-Sadi, Rana;Guo, Shuhong;Ma, Thomas Y.
通讯作者：
Ma, Thomas Y.
影响因子：
3.7
作者：
Choung, Rok Seon;Locke, G. Richard, III
通讯作者：
Locke, G. Richard, III