Comprehensive allelotyping of human intrahepatic cholangiocarcinoma.
Comprehensive allelotyping of human intrahepatic cholangiocarcinoma.
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人肝内胆管癌的综合等位基因型。
DOI：
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发表时间：
2001
影响因子：
11.5
通讯作者：
M. Fukumoto
中科院分区：
文献类型：
--
作者：
H. Momoi;H. Okabe;T. Kamikawa;S. Satoh;I. Ikai;M. Yamamoto;A. Nakagawara;Y. Shimahara;Y. Yamaoka;M. Fukumoto
我们对22例肝内胆管细胞癌(ICC)进行了全基因组杂合性缺失(LOH)扫描，使用168个分布于所有人类染色体的多态微卫星标记和48个LOH标记，其中LOH是肝细胞癌(HCC)的特征。在超过30%的信息性病例中，在21个基因座上观察到了LOH的标记。其中，6q(三个座位)、4q(两个座位)、9q、16q和17p上的8个标记与我们先前研究中发现的肝细胞癌具有相同的高频率LOH。在大体形态上，肿块型肿瘤与其他类型相比有较高的LOH发生率(P<；0.001)。比较肿瘤大小(或=5厘米与5厘米)、数目(多发与单发)和国际抗癌联合会TNM分类(IVB期与II-IVA期)，LOH在晚期更常见(P<；0.01)。然而，杂合性缺失的频率与淋巴结状态无关(pN0与PN1)。在无淋巴结转移的大肿瘤中，包括p73基因在内的1p36上频繁的杂合性缺失被发现。提示ICC与肝细胞癌有共同的致癌步骤，如4q和6q的LOH，染色体1p36上抑癌基因的失活与ICC的进展有关，而与转移无关。
We performed a genome-wide scan for loss of heterozygosity (LOH) in 22 intrahepatic cholangiocarcinoma (ICC) cases using 168 polymorphic microsatellite markers throughout all of the human chromosomes and 48 markers of which LOH is reportedly characteristic of hepatocellular carcinoma (HCC). Markers with LOH in more than 30% of informative cases were observed at 21 loci. Among these, eight markers on 6q (three loci), 4q (two loci), 9q, 16q, and 17p shared high frequencies of LOH with HCC in our previous study. As for gross appearance, mass-forming type tumors showed higher frequency of LOH (P < 0.001) compared with other types. Compared by tumor size (< or =5 cm versus >5 cm), number (multiple versus solitary), and the International Union Against Cancer TNM classification (stage IVB versus II-IVA), LOH was observed more frequently in advanced stages (P < 0.01, respectively). However, LOH frequency does not differ regardless of lymph node status (pN0 versus pN1). Frequent LOH on 1p36 including the p73 locus was noted in large tumors without lymph node metastasis. These suggest that ICC shares some common carcinogenic steps with HCC such as LOH of 4q and 6q and that inactivation of tumor suppressor genes on chromosome 1p36 contributes to progression of ICC but not to metastatic traits.
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影响因子：
8
作者：
A. T. D. Souza;G. R. Hankins;M. Washington;R. Fine;T. Orton;R. Jirtle
通讯作者：
A. T. D. Souza;G. R. Hankins;M. Washington;R. Fine;T. Orton;R. Jirtle
DOI：
10.1073/pnas.89.21.10557
发表时间：
1992-11-01
影响因子：
11.1
作者：
FOUNTAIN, JW;KARAYIORGOU, M;DRACOPOLI, NC
通讯作者：
DRACOPOLI, NC
影响因子：
11.2
作者：
A. Coleman;J. Fountain;T. Nobori;O. Olopade;Gavin P. Robertson;D. Housman;T. Lugo
通讯作者：
A. Coleman;J. Fountain;T. Nobori;O. Olopade;Gavin P. Robertson;D. Housman;T. Lugo
影响因子：
56.9
作者：
KAMB, A;GRUIS, NA;SKOLNICK, MH
通讯作者：
SKOLNICK, MH
DOI：
10.1073/pnas.91.21.9871
发表时间：
1994-10-11
影响因子：
11.1
作者：
MAO, L;LEE, DJ;SIDRANSKY, D
通讯作者：
SIDRANSKY, D