Dynamic evolution of left ventricular strain and microvascular perfusion assessed by speckle tracking echocardiography and myocardial contrast echocardiography in diabetic rats: Effect of dapagliflozin.
Dynamic evolution of left ventricular strain and microvascular perfusion assessed by speckle tracking echocardiography and myocardial contrast echocardiography in diabetic rats: Effect of dapagliflozin.
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DOI：
10.3389/fcvm.2023.1109946
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发表时间：
2023
影响因子：
3.6
通讯作者：
Shen, Ying
中科院分区：
文献类型：
--
作者：
Liu, Juan;Wang, Yixuan;Zhang, Jun;Li, Xin;Tan, Lin;Huang, Haiyun;Dai, Yang;Shang, Yongning;Shen, Ying
关键词：
本实验旨在通过综合超声心动图检测糖尿病大鼠心肌应变和微血管灌注的动态变化，同时评价达格列净（达帕）的作用。雄性Sprague-Dawley大鼠（n = 128）基于存在或不存在高脂肪饮食和链脲佐菌素诱导的糖尿病（有或没有达帕治疗）被随机分成四组（n = 32/组）。分别于2、4、6、8周行常规超声、二维斑点追踪超声心动图（2D-STE）和心肌声学造影（MCE）检查，测定左室整体纵向应变（GLS）、心肌血流速度（MBFV）、心肌血流量（MBF）和心肌血容量（MBV）。在最后一次超声测量后立即处死所有动物，进行组织病理学评估。尽管2、4、6、8周时各组的常规多普勒超声指标相似（p > 0.05），但2D-STE和MCE检测到糖尿病大鼠8周时左室GLS、MBFV、MBF和MBV均降低（p < 0.05）。在用达帕治疗糖尿病大鼠后6周和8周，这些指标显著改善（p < 0.05），达到在非糖尿病对照中观察到的类似值。达帕治疗与糖尿病大鼠心肌空泡化和微血管密度增加以及间质纤维化减少相关。2D-STE和MCE联合应用对糖尿病前期和糖尿病早期的左室畸形和微血管灌注受损具有较高的敏感性。达帕对糖尿病大鼠心肌灌注有保护作用。
This experimental study aimed to determine the dynamic changes in myocardial strain and microvascular perfusion in diabetic rats by comprehensive echocardiography while evaluating the effect of dapagliflozin (DAPA). Male Sprague–Dawley rats (n = 128) were randomly divided into four groups based on the presence or absence of a high-fat diet and streptozotocin-induced diabetes with or without DAPA treatment (n = 32/group). Serial conventional ultrasound, two-dimensional speckle tracking echocardiography (2D-STE) and myocardial contrast echocardiography (MCE) were performed at 2, 4, 6, and 8 weeks, and left ventricular global longitudinal strain (GLS), myocardial blood flow velocity (MBFV), myocardial blood flow (MBF), and myocardial blood volume (MBV) were determined. All animals were sacrificed immediately after the last echo measurement for histopathological assessment. Despite similar conventional Doppler-echo indexes among the groups at 2, 4, 6, and 8 weeks (p > 0.05), left ventricular GLS, MBFV, MBF, and MBV were decreased at 8 weeks in diabetic rats (p < 0.05) as detected by both 2D-STE and MCE. These indexes were significantly improved at 6 and 8 weeks after treatment with DAPA for diabetic rats (p < 0.05), reaching similar values observed in non-diabetic controls. DAPA treatment was associated with increased myocardial vacuolization and microvessel density and reduced interstitial fibrosis in diabetic rats. Combined 2D-STE and MCE is sensitive for detecting left ventricular deformity and impaired microvascular perfusion in prediabetes and the early stage of diabetes mellitus. DAPA exerts a beneficial effect on protecting myocardial perfusion in diabetic rats.
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影响因子：
3.6
作者：
Vink CEM;van de Hoef TP;Götte MJW;Eringa EC;Appelman Y
通讯作者：
Appelman Y
影响因子：
1.9
作者：
Huang X;Yue Y;Wang Y;Deng Y;Liu L;Di Y;Sun S;Chen D;Fan L;Cao J
通讯作者：
Cao J
DOI：
10.1152/ajpendo.00158.2022
发表时间：
2022-12-01
影响因子：
5.1
作者：
Sadana，Prabodh;Edler，Melissa;Kasumov，Takhar
通讯作者：
Kasumov，Takhar
影响因子：
158.5
作者：
Solomon, S. D.;McMurray, J. J., V;Langkilde, A. M.
通讯作者：
Langkilde, A. M.
影响因子：
9.3
作者：
Cosyns B;Droogmans S;Hernot S;Degaillier C;Garbar C;Weytjens C;Roosens B;Schoors D;Lahoutte T;Franken PR;Van Camp G
通讯作者：
Van Camp G