Unexpected Discovery of Hypermutator Phenotype Sounds the Alarm for Quality Control Strains.
Unexpected Discovery of Hypermutator Phenotype Sounds the Alarm for Quality Control Strains.
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DOI：
10.1093/gbe/evab148
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发表时间：
2021-08-03
影响因子：
3.3
通讯作者：
Long H
中科院分区：
文献类型：
--
作者：
Wu K;Cheng ZH;Williams E;Turner NT;Ran D;Li H;Zhou X;Guo H;Sung W;Liu DF;Lynch M;Long H
Microbial strains with high genomic stability are particularly sought after for testing the quality of commercial microbiological products, such as biological media and antibiotics. Yet, using mutation–accumulation experiments and de novo assembled complete genomes based on Nanopore long-read sequencing, we find that the widely used quality-control strain Shewanella putrefaciens ATCC-8071, also a facultative pathogen, is a hypermutator, with a base-pair substitution mutation rate of 2.42 × 10−8 per nucleotide site per cell division, ∼146-fold greater than that of the wild-type strain CGMCC-1.6515. Using complementation experiments, we confirm that mutL dysfunction, which was a recent evolutionary event, is the cause for the high mutation rate of ATCC-8071. Further analyses also give insight into possible relationships between mutation and genome evolution in this important bacterium. This discovery of a well-known strain being a hypermutator necessitates screening the mutation rate of bacterial strains before any quality control or experiments.
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影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
DOI：
10.1073/pnas.96.5.1971
发表时间：
1999-03-02
影响因子：
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作者：
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通讯作者：
Squires, CL
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.5
作者：
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通讯作者：
Guindon, Stephane