Intracellular localization and metabolism of DNA polymerase alpha in human cells visualized with monoclonal antibody.
Intracellular localization and metabolism of DNA polymerase alpha in human cells visualized with monoclonal antibody.
复制标题
使用单克隆抗体可视化人类细胞中 DNA 聚合酶 α 的细胞内定位和代谢。
DOI：
10.1016/0014-4827(84)90362-8
复制
发表时间：
1984
影响因子：
3.7
通讯作者：
S. Yoshida
中科院分区：
文献类型：
--
作者：
H. Nakamura;T. Morita;S. Masaki;S. Yoshida
用抗DNA聚合酶α单克隆抗体间接免疫荧光显微镜观察发现，DNA聚合酶α在转化的人细胞的G1、S和G2期位于核内，在有丝分裂过程中分散分布在胞质中。在正常人皮肤成纤维细胞或淋巴细胞的静止期（G 0期），α-酶几乎不能通过免疫荧光或酶活性检测到。然而，通过将细胞暴露于增殖刺激，DNA聚合酶a在DNA合成开始之前出现在细胞核中，在S期期间迅速增加，在S期和G2期晚期达到最高水平，然后通过有丝分裂重新分配到子细胞。还发现增殖刺激引起的DNA聚合酶a量的增加不受用阿非迪霉素或羟基脲抑制DNA合成的影响。
Indirect immunofluorescence microscopy with monoclonal antibody against DNA polymerase α revealed the intranuclear localization of DNA polymerase α in G1, S, and G2 phases of transformed human cells, and dispersed cytoplasmic distribution during mitosis. In the quiescent, G0 phase of normal human skin fibroblasts or lymphocytes, the α-enzyme was barely detectable by either immunofluorescence or enzyme activity. By exposing cells to proliferation stimuli, however, DNA polymerase a appeared in the nuclei just prior to onset of DNA synthesis, increased rapidly during S phase, reached the maximum level at late S and G2 phases, and was then redistributed to the daughter cells through mitosis. It was also found that the increase in the amount of DNA polymerase a by proliferation stimuli was not affected by inhibition of DNA synthesis with aphidicolin or hydroxyurea.
影响因子：
2.9
作者：
H. C. Smith;R. Berezney
通讯作者：
H. C. Smith;R. Berezney
DOI：
10.1016/s0021-9258(18)34344-8
发表时间：
1982-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
K. Bensch;Shoichiro Tanaka;S. Z. Hu;T. S. Wang;D. Korn
通讯作者：
K. Bensch;Shoichiro Tanaka;S. Z. Hu;T. S. Wang;D. Korn