Genetic Diversity of Cameroon Cattle and a Putative Genomic Map for Resistance to Bovine Tuberculosis.
Genetic Diversity of Cameroon Cattle and a Putative Genomic Map for Resistance to Bovine Tuberculosis.
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DOI：
10.3389/fgene.2020.550215
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发表时间：
2020
影响因子：
3.7
通讯作者：
Muwonge A
中科院分区：
文献类型：
--
作者：
Callaby R;Kelly R;Mazeri S;Egbe F;Benedictus L;Clark E;Doeschl-Wilson A;Bronsvoort B;Salavati M;Muwonge A
牛结核是由牛分枝杆菌引起的一种具有全球性经济和公共卫生意义的畜禽疾病。目前没有有效的牲畜疫苗，因此控制依赖于动物水平的监测和乳制品的巴氏杀菌。一种新的替代控制方法是利用宿主的遗传变异性；最近的研究表明，培育欧洲牛磺酸牛，如Holsteins，以提高对BTB的抗性是可行的。这种方法对非洲牛种群的效用仍然是未知的。这项研究旨在评估喀麦隆牛对牛结核病抗性的遗传变异和潜在的基因组结构。我们对喀麦隆2346头被屠宰的牛进行了横断面研究。采集咽后淋巴结标本，在Lowenstein Jensen培养基和BACTEC MGIT 960系统上培养，用Hain®基因分型试剂盒鉴定牛分枝杆菌。共有153头牛的牛分枝杆菌呈阳性，并与随机选择的阴性样本一起存档。在这项研究中，我们对212头牛的存档样本进行了基因分型。采用主成分分析、系统聚类和混合分析等方法对它们的基因组多样性进行了分析。通过遗传力分析和数量性状基因座的比较分析，评估了小麦抗白叶枯病的遗传变异。之前对这一研究群体的研究表明，富拉尼牛比混合品种的牛更容易感染牛结核病。然而，我们在这里表明，这些明显的表型差异并不反映在明显的基因组差异上。在遗传水平上，富拉尼牛和混合牛都表现出类似的混合模式，并有牛磺酸和吲哚祖先的证据。在被研究的人群中，几乎没有欧洲牛磺酸的渗透。等级聚类显示了牛的集群，它们对牛结核病的易感性不同。我们的发现暗示BTB耐药性是多基因的。这项研究强调了非洲牛结核病遗传控制的潜力，以及进一步研究非洲牛种群中牛结核病耐药性的遗传学的必要性。
Bovine Tuberculosis (bTB) caused by Mycobacterium bovis is a livestock disease of global economic and public health importance. There are currently no effective vaccines available for livestock and so control relies on animal level surveillance and pasteurization of dairy products. A new alternative control approach is to exploit the genetic variability of the host; recent studies have demonstrated that breeding European taurine cattle, such as Holsteins for increased resistance to bTB is feasible. The utility of such an approach is still unknown for African cattle populations. This study aims to assess genetic variation in bTB resistance and the underlying genomic architecture of cattle in Cameroon. We conducted a cross-sectional study of 2,346 slaughter cattle in Cameroon. Retropharyngeal lymph node samples were collected and cultured on Lowenstein Jensen media and the BACTEC MGIT 960 system, and M. bovis was identified using the Hain® Genotype kits. A total of 153 cattle were positive for M. bovis and were archived along with a random selection of negative samples. In this study, we genotyped archived samples from 212 cattle. Their genomic diversity was characterized using PCA, hierarchical clustering and admixture analysis. We assessed genetic variation in bTB resistance using heritability analysis and compared quantitative trait loci. Previous research on this study population have shown that Fulani cattle are more susceptible to bTB than mixed breeds. However, here we show that these apparent phenotypic differences in breeds are not reflected by clear genomic differences. At the genetic level, both the Fulani and mixed cattle show similar patterns of admixture with evidence of both taurine and indicine ancestry. There was little European taurine introgression within the studied population. Hierarchical clustering showed clusters of cattle that differed in their susceptibility to bTB. Our findings allude to bTB resistance being polygenic in nature. This study highlights the potential for genetic control of bTB in Africa and the need for further research into the genetics of bTB resistance within African cattle populations.
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DOI：
10.1016/j.fsigen.2016.03.004
发表时间：
2016-07
期刊：
Forensic science international. Genetics
影响因子：
--
作者：
Buckleton J;Curran J;Goudet J;Taylor D;Thiery A;Weir BS
通讯作者：
Weir BS
影响因子：
4.6
作者：
Egbe NF;Muwonge A;Ndip L;Kelly RF;Sander M;Tanya V;Ngwa VN;Handel IG;Novak A;Ngandalo R;Mazeri S;Morgan KL;Asuquo A;Bronsvoort BM
通讯作者：
Bronsvoort BM
影响因子：
3.7
作者：
Bahbahani H;Tijjani A;Mukasa C;Wragg D;Almathen F;Nash O;Akpa GN;Mbole-Kariuki M;Malla S;Woolhouse M;Sonstegard T;Van Tassell C;Blythe M;Huson H;Hanotte O
通讯作者：
Hanotte O
影响因子：
9.2
作者：
Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
Lee JJ
影响因子：
3.5
作者：
Bermingham, M. L.;More, S. J.;Berry, D. P.
通讯作者：
Berry, D. P.