Cloning and expression of human corneal calgranulin C (CO-Ag).
Cloning and expression of human corneal calgranulin C (CO-Ag).
复制标题
人角膜钙颗粒蛋白 C (CO-Ag) 的克隆和表达。
DOI：
10.1076/ceyr.17.9.870.5136
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发表时间：
1998
影响因子：
2
通讯作者：
Liu,SH
中科院分区：
文献类型：
--
作者：
Gottsch,JD;Liu,SH
PURPOSEA host-parasite interaction is thought to be involved in the pathogenesis of Mooren's ulcer. We have identified a cornea-associated antigen (CO-Ag), which may be a target for the autoimmune process resulting in Mooren's ulcer. This study presents the cloning, expression, and identification of a cDNA encoding human CO-Ag.METHODSReverse transcription-polymerase chain reaction (RT-PCR) was performed to amplify a cDNA encoding CO-Ag in the human cornea. The cDNA fragment was cloned into a prokaryotic expression vector and the resulting plasmid was transformed into DH5 E. coli cells. Autoantibody reactivity to the CO-Ag fusion protein in patient sera was tested by Western blots.RESULTSA cDNA encoding human CO-Ag was amplified by RT-PCR. The entire mRNA coding region was 273 nucleotides in length, predicting a 91-amino acid protein with a molecular weight of 10,683 daltons. The cDNA sequence was identical to human neutrophil calgranulin C (CaGC). Human CO-Ag was expressed in E. coli carrying a plasmid in which the CO-Ag cDNA was under control of the E. coli trc promoter. The CO-Ag fusion protein, which comprised as much as 15% of the total bacterial protein, was purified to 90% homogeneity by affinity chromatography on an immobilized metal column. The recombinant CO-Ag protein produced was recognized by auto-antibodies in the sera of 6 of 15 patients with Mooren's ulcer and none of 14 normal control sera by Western blots.CONCLUSIONCO-Ag is identical to calgranulin C, a neutrophil protein found on the surface of filarial nematodes. A host-parasite interaction may cause autoimmunity to CO-Ag (CaGC) in the cornea resulting in a Mooren's ulcer.
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影响因子：
4.2
作者：
KIETZMAN, B
通讯作者：
KIETZMAN, B
人类防御素自身抗体：与高反应性盘尾丝虫病 (sowda) 的疾病相关性
DOI：
10.1016/0169-4758(95)80183-9
发表时间：
1995
期刊：
The Journal of Experimental Medicine
影响因子：
--
作者：
M. Gallin;Anke B. Jacobi;Dietrich W. Biittner;Oyvind Sch;Thomas Marti;K. Erttmann
通讯作者：
K. Erttmann
影响因子：
4.2
作者：
GOTTSCH, JD;LIU, SH;STARK, WJ
通讯作者：
STARK, WJ
牛角膜抗原cDNA的克隆和表达。
影响因子：
2
作者：
Gottsch,JD;Liu,SH
通讯作者：
Liu,SH
脑细胞外液的蛋白质：S-100 蛋白释放的证据
影响因子：
4.7
作者：
V. Shashoua;G. Hesse;B. Moore
通讯作者：
B. Moore