pH-controlled DNA- and RNA-templated assembly of short oligomers.
pH-controlled DNA- and RNA-templated assembly of short oligomers.
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DOI：
10.1039/c4sc03028a
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发表时间：
2015-01-01
期刊：
影响因子：
8.4
通讯作者：
Smietana M
中科院分区：
文献类型：
--
作者：
Barbeyron R;Vasseur JJ;Smietana M
报道了一种无刺激、无酶、无激活剂、以DNA和RNA为模板的双功能短寡核苷酸组装。在人工遗传学领域，合成构件的非酶自组织的发展对于提供具有扩展功能的生物聚合物和了解益生菌过程都是至关重要的。虽然可逆性被认为在早期功能遗传物质中发挥了重要作用，但我们之前报道了一种有效的DNA模板连接适当设计的5‘端硼酸和3’端核糖核苷半序列。在这里，我们报告了无酶和无激活的DNA和RNA模板的双功能六聚体的组装。可逆组装被发现是区域和序列特异性的，并将得到的双链的稳定性与它们的缺口对应物进行了比较。为了进一步了解这一史无前例的过程，我们还研究了一种自动模板化的双链自组装，这是朝着序列定义的合成聚合物进化的关键一步。
A stimuli-responsive enzyme-free and activator-free DNA- and RNA-templated assembly of bifunctional short oligonucleotides is reported. In the area of artificial genetics the development of non-enzymatic self-organization of synthetic building blocks is critical for both providing biopolymers with extended functions and understanding prebiotic processes. While reversibility is believed to have played a major role in early functional genetic materials, we previously reported an efficient DNA-templated ligation of suitably designed 5′-end boronic acid and 3′-end ribonucleosidic half-sequences. Here, we report the enzyme-free and activation-free DNA- and RNA-templated assembly of bifunctional hexamers. The reversible assembly was found to be regio- and sequence specific and the stabilities of the resulting duplexes were compared to their nicked counterparts. To go further with our understanding of this unprecedented process we also examined an auto-templated duplex self-assembly representing a key step toward the evolution of sequence-defined synthetic polymers.
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影响因子：
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