Real-time dynamic imaging of virus distribution in vivo.
Real-time dynamic imaging of virus distribution in vivo.
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DOI：
10.1371/journal.pone.0017076
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发表时间：
2011-02-15
期刊：
影响因子：
3.7
通讯作者：
Barry MA
中科院分区：
文献类型：
--
作者：
Hofherr SE;Adams KE;Chen CY;May S;Weaver EA;Barry MA
病毒和基因治疗载体的分布很难在活的生物体中评估。例如，鼠模型中的贩运通常只能在处死动物进行组织切片或提取后进行评估。这些测定是费力的，需要整个动物切片以确定组织定位。他们还能够在一只动物身上进行纵向或动力学研究。为了在全身感染后追踪病毒，我们用近红外（NIR）荧光团标记了腺病毒，并在小鼠静脉注射后对其进行成像。成像能够跟踪和定量病毒颗粒在注射的同时进入颈静脉，在500毫秒内出现在心脏中，在7秒内分布在血流中和整个动物中，并且大部分病毒分布基本上在3分钟内完成。这些数据首次提供了对系统性病毒感染的快速初始事件的体内实时跟踪。
The distribution of viruses and gene therapy vectors is difficult to assess in a living organism. For instance, trafficking in murine models can usually only be assessed after sacrificing the animal for tissue sectioning or extraction. These assays are laborious requiring whole animal sectioning to ascertain tissue localization. They also obviate the ability to perform longitudinal or kinetic studies in one animal. To track viruses after systemic infection, we have labeled adenoviruses with a near-infrared (NIR) fluorophore and imaged these after intravenous injection in mice. Imaging was able to track and quantitate virus particles entering the jugular vein simultaneous with injection, appearing in the heart within 500 milliseconds, distributing in the bloodstream and throughout the animal within 7 seconds, and that the bulk of virus distribution was essentially complete within 3 minutes. These data provide the first in vivo real-time tracking of the rapid initial events of systemic virus infection.
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影响因子：
46.9
作者：
Douglas, JT;Rogers, BE;Curiel, DT
通讯作者：
Curiel, DT
影响因子：
20.3
作者：
Parker, Alan L.;Waddington, Simon N.;Baker, Andrew H.
通讯作者：
Baker, Andrew H.
DOI：
10.1007/s00259-007-0578-z
发表时间：
2008-02-01
影响因子：
9.1
作者：
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通讯作者：
Lucignani, Giovanni
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3.5
作者：
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通讯作者：
Sevick-Muraca, Eva M.
影响因子：
5.4
作者：
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通讯作者：
Kay, MA