The nasal microbiome, nasal transcriptome, and pet sensitization.
The nasal microbiome, nasal transcriptome, and pet sensitization.
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DOI：
10.1016/j.jaci.2021.01.031
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
Bunyavanich S
中科院分区：
文献类型：
--
作者：
Chun Y;Do A;Grishina G;Arditi Z;Ribeiro V;Grishin A;Vicencio A;Bunyavanich S
宠物过敏在哮喘儿童中很常见。微生物群和宿主反应可介导过敏原致敏。通过鼻微生物组和鼻转录组的联合检查，揭示宠物过敏原致敏中的宿主-微生物关系。我们收集了132名哮喘儿童的鼻腔样本，进行平行的16 S rRNA和RNA测序。测量猫和狗皮屑的特异性IgE水平。针对宠物致敏状态，对鼻微生物组、鼻转录组及其相关性进行分析。在132名儿童中，91名（68.9%）为猫致敏，96名（72.7%）为狗致敏。猫致敏与Shannon指数（P=0.021）和加权UniFrac距离（PERMANOVA P=0.035）的鼻内细菌组成差异的鼻内微生物多样性降低相关。棒状杆菌属和表皮葡萄球菌的丰度明显较低，宠物致敏儿童的代谢过程“线粒体中脂肪酸的延长”较低。相关网络显示，鼻表达水平的47个基因代表炎症过程与棒状杆菌属和表皮葡萄球菌的相对丰度呈负相关。因此，这些物种不仅与宠物致敏的缺乏直接相关，而且与导致过敏原致敏的炎症过程的宿主基因表达的低表达相关。因果中介分析表明，这些鼻物种和宠物致敏之间的关联是由鼻基因表达介导的。鼻棒状杆菌和表皮葡萄球菌的高丰度与宠物致敏的缺乏相关，并与炎症基因的低表达相关。鼻微生物群和鼻基因表达因宠物致敏状态而异。鼻棒状杆菌属和表皮葡萄球菌的高丰度与宠物致敏的缺乏相关，并与炎症基因的较低宿主表达相关。
Pet allergies are common in children with asthma. Microbiota and host responses may mediate allergen sensitization. To uncover host-microbe relationships in pet allergen sensitization via joint examination of the nasal microbiome and nasal transcriptome. We collected nasal samples from 132 children with asthma for parallel 16S rRNA and RNA sequencing. Specific IgE levels for cat and dog dander were measured. Analyses of the nasal microbiome, nasal transcriptome, and their correlations were performed with respect to pet sensitization status. Among the 132 children, 91 (68.9%) were cat sensitized and 96 (72.7%) were dog sensitized. Cat sensitization was associated with lower nasal microbial diversity by Shannon Index (P=0.021) and differential nasal bacterial composition by weighted UniFrac distance (PERMANOVA P=0.035). Corynebacterium sp. and Staphylococcus epidermidis were significantly less abundant, and the metabolic process “fatty acid elongation in mitochondria” was lower in pet sensitized vs. unsensitized children. Correlation networks revealed that the nasal expression levels of 47 genes representing inflammatory processes were negatively correlated with the relative abundances of Corynebacterium sp. and Staphylococcus epidermidis. Thus, these species were not only directly associated with the absence of pet sensitization, but also with the under-expression of host gene expression of inflammatory processes that contribute to allergen sensitization. Causal mediation analyses revealed that the associations between these nasal species and pet sensitization were mediated by nasal gene expression. Higher abundances of nasal Corynebacterium sp. and Staphylococcus epidermidis are associated with absence of pet sensitization and correlate with lower expression of inflammatory genes. Nasal microbiota and nasal gene expression vary by pet sensitization status. Higher abundances of nasal Corynebacterium sp. and Staphylococcus epidermidis are associated with absence of pet sensitization and correlate with lower host expression of inflammatory genes.
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