Permeability changes of integrin-containing multivesicular structures triggered by picornavirus entry.
Permeability changes of integrin-containing multivesicular structures triggered by picornavirus entry.
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DOI：
10.1371/journal.pone.0108948
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cheng RH
中科院分区：
文献类型：
--
作者：
Soonsawad P;Paavolainen L;Upla P;Weerachatyanukul W;Rintanen N;Espinoza J;McNerney G;Marjomäki V;Cheng RH
聚集的α2β1-整合素的细胞摄取诱导形成膜室，随后成熟为多泡体（MVB）。在感染小核糖核酸echovirus 1 （EVI）后，观察到聚集整合素介导的内化增强。我们通过在病毒早期侵入时对细胞进行高压冷冻固定，然后进行免疫电子断层扫描，阐明了病毒诱导的MVBs （vMVBs）与抗体诱导的对照MVBs（模拟感染）的结构特征。三维断层扫描显示，在感染后2和3.5小时，与未感染病毒的对照MVBs相比，这些vMVBs和腔内囊泡（ILVs）的大小和复杂性显着增加。2 h后，特别是3.5 h后，从断层扫描中可以检测到vMVBs膜上的断裂，这表明这些断裂有助于基因组释放到细胞质中。病毒基因组的原位中性红标记表明，病毒脱壳早在30分钟开始，而基于共聚焦显微镜检测，vMVBs的渗透性在1至3小时之间增加。总之，这些数据表明，在这种非包膜肠病毒的感染进入途径中，核内体的大小和通透性发生了显著的形态学变化，并表明形成的断裂有助于将基因组转移到细胞质中进行复制。
Cellular uptake of clustered α2β1-integrin induces the formation of membrane compartments that subsequently mature into a multivesicular body (MVB). Enhanced internalization mediated by clustered integrins was observed upon infection by the picornavirus echovirus 1 (EVI). We elucidated the structural features of virus-induced MVBs (vMVBs) in comparison to antibody-induced control MVBs (mock infection) by means of high-pressure cryo fixation of cells followed by immuno electron tomography during early entry of the virus. Three-dimensional tomograms revealed a marked increase in the size and complexity of these vMVBs and the intraluminal vesicles (ILVs) at 2 and 3.5 hours post infection (p.i.), in contrast to the control MVBs without virus. Breakages in the membranes of vMVBs were detected from tomograms after 2 and especially after 3.5 h suggesting that these breakages could facilitate the genome release to the cytoplasm. The in situ neutral-red labeling of viral genome showed that virus uncoating starts as early as 30 min p.i., while an increase of permeability was detected in the vMVBs between 1 and 3 hours p.i., based on a confocal microscopy assay. Altogether, the data show marked morphological changes in size and permeability of the endosomes in the infectious entry pathway of this non-enveloped enterovirus and suggest that the formed breakages facilitate the transfer of the genome to the cytoplasm for replication.
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影响因子：
9.8
作者：
Brandenburg B;Lee LY;Lakadamyali M;Rust MJ;Zhuang X;Hogle JM
通讯作者：
Hogle JM
DOI：
10.1083/jcb.147.2.401
发表时间：
1999-10-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ivaska J;Reunanen H;Westermarck J;Koivisto L;Kähäri VM;Heino J
通讯作者：
Heino J
影响因子：
14.8
作者：
Slot, Jan W.;Geuze, Hans J.
通讯作者：
Geuze, Hans J.
影响因子：
3.3
作者：
Karjalainen, Mikko;Kakkonen, Elina;Marjomaki, Varpu
通讯作者：
Marjomaki, Varpu
影响因子：
5.4
作者：
Soonsawad, Pan;Xing, Li;Cheng, R. Holland
通讯作者：
Cheng, R. Holland