MicroRNA-146a expresses in interleukin-17 producing T cells in rheumatoid arthritis patients.
MicroRNA-146a expresses in interleukin-17 producing T cells in rheumatoid arthritis patients.
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DOI：
10.1186/1471-2474-11-209
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发表时间：
2010-09-15
影响因子：
2.3
通讯作者：
Ochi M
中科院分区：
文献类型：
--
作者：
Niimoto T;Nakasa T;Ishikawa M;Okuhara A;Izumi B;Deie M;Suzuki O;Adachi N;Ochi M
白细胞介素（IL）-17是类风湿关节炎（RA）发病机制中的重要因素。微小RNA（microRNA，miRNA）是一类非编码RNA家族，与类风湿关节炎等人类疾病密切相关。本研究的目的是鉴定IL-17产生细胞分化中的miRNA，并分析其在RA患者外周血单个核细胞（PBMC）和滑膜中的表达模式。从CD 4 +T细胞扩增产生IL-17的细胞。利用miRNA芯片技术鉴定IL-17产生细胞分化过程中的miRNA。定量聚合酶链反应检测RA和骨关节炎（OA）患者PBMC和滑膜中所鉴定的miRNA的表达模式。采用原位杂交和IL-17免疫组化双重染色方法，分析所鉴定的miRNA在滑膜中的表达模式。6种miRNAs let-7a、miR-26、miR-146 a/B、miR-150和miR-155在产生IL-17的T细胞中显著上调。在Larsen分级低、病程短的患者中，miR-146 a和IL-17的表达高于PBMC。与OA相比，miR-146 a在RA滑膜中强烈表达。MiR-146 a在滑膜组织中强烈表达，在疾病活动度高的患者滑膜组织中IL-17高表达。双重染色显示miR-146 a在IL-17表达细胞中表达。这些结果表明，miR-146 a与RA患者PBMC和滑膜中IL-17的表达相关。miR-146 a可能参与IL-17的表达。
Interleukin (IL)-17 is an important factor in rheumatoid arthritis (RA) pathogenesis. MicroRNA (miRNA)s are a family of non coding RNAs and associated with human diseases including RA. The purpose of this study is to identify the miRNAs in the differentiation of IL-17 producing cells, and analyze their expression pattern in the peripheral blood mononuclear cells (PBMC) and synovium from RA patients. IL-17 producing cells were expanded from CD4+T cell. MiRNA microarray was performed to identify the miRNAs in the differentiation of IL-17 producing cells. Quantitative polymerase chain reaction was performed to examine the expression patterns of the identified miRNAs in the PBMC and synovium from RA and osteoarthritis (OA) patients. Double staining combining in situ hybridization and immunohistochemistry of IL-17 was performed to analyze the expression pattern of identified miRNA in the synovium. Six miRNAs, let-7a, miR-26, miR-146a/b, miR-150, and miR-155 were significantly up regulated in the IL-17 producing T cells. The expression of miR-146a and IL-17 was higher than in PBMC in the patients with low score of Larsen grade and short disease duration. MiR-146a intensely expressed in RA synovium in comparison to OA. MiR-146a expressed intensely in the synovium with hyperplasia and high expression of IL-17 from the patients with high disease activity. Double staining revealed that miR-146a expressed in IL-17 expressing cells. These results indicated that miR-146a was associated with IL-17 expression in the PBMC and synovium in RA patients. There is the possibility that miR-146a participates in the IL-17 expression.
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影响因子：
64.5
作者：
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通讯作者：
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