Inhibition of Zika Virus Replication by Silvestrol.
Inhibition of Zika Virus Replication by Silvestrol.
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DOI：
10.3390/v10040149
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发表时间：
2018-03-27
期刊：
影响因子：
--
通讯作者：
Hildt E
中科院分区：
文献类型：
--
作者：
Elgner F;Sabino C;Basic M;Ploen D;Grünweller A;Hildt E
2016年在南美洲爆发的寨卡病毒（ZIKV）具有特定的致病性结果，突出了对具有广谱活性的新型抗病毒物质的需求，以快速应对新出现的病毒病原体的意外爆发。最近，从植物米仔兰中分离的天然化合物silvestrol被发现对（-）-链RNA病毒埃博拉病毒以及具有（+）-链RNA基因组的冠状病毒和小核糖核酸病毒具有非常有效的抗病毒作用。这种抗病毒活性是基于通过抑制DEAD盒RNA解旋酶真核起始因子-4A（eIF 4A）而损害病毒RNA的翻译，该因子是解开几种原癌基因的结构化5 ′-非翻译区（5′-UTR）所必需的，从而促进其翻译。寨卡病毒是一种黄病毒，具有正链RNA基因组，含有具有不同二级结构元件的5′-加帽UTR。因此，我们研究了司维司群对A549细胞和原代人肝细胞中ZIKV复制的影响。使用两种不同的ZIKV毒株。在感染的A549细胞和原代人肝细胞中，司维司群具有对两种分析的菌株发挥ZIKV复制的显著抑制的潜力，即使来自乌干达的祖先菌株对司维司群不太敏感。我们的数据可能有助于确定参与控制ZIKV感染的宿主因素，并有助于开发可用于治疗各种病毒感染而没有耐药性风险的抗病毒概念，因为宿主蛋白是靶向的。
The Zika virus (ZIKV) outbreak in 2016 in South America with specific pathogenic outcomes highlighted the need for new antiviral substances with broad-spectrum activities to react quickly to unexpected outbreaks of emerging viral pathogens. Very recently, the natural compound silvestrol isolated from the plant Aglaia foveolata was found to have very potent antiviral effects against the (−)-strand RNA-virus Ebola virus as well as against Corona- and Picornaviruses with a (+)-strand RNA-genome. This antiviral activity is based on the impaired translation of viral RNA by the inhibition of the DEAD-box RNA helicase eukaryotic initiation factor-4A (eIF4A) which is required to unwind structured 5´-untranslated regions (5′-UTRs) of several proto-oncogenes and thereby facilitate their translation. Zika virus is a flavivirus with a positive-stranded RNA-genome harboring a 5′-capped UTR with distinct secondary structure elements. Therefore, we investigated the effects of silvestrol on ZIKV replication in A549 cells and primary human hepatocytes. Two different ZIKV strains were used. In both infected A549 cells and primary human hepatocytes, silvestrol has the potential to exert a significant inhibition of ZIKV replication for both analyzed strains, even though the ancestor strain from Uganda is less sensitive to silvestrol. Our data might contribute to identify host factors involved in the control of ZIKV infection and help to develop antiviral concepts that can be used to treat a variety of viral infections without the risk of resistances because a host protein is targeted.
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DOI：
10.1128/genomea.00500-14
发表时间：
2014-06-05
期刊：
Genome announcements
影响因子：
--
作者：
Baronti C;Piorkowski G;Charrel RN;Boubis L;Leparc-Goffart I;de Lamballerie X
通讯作者：
de Lamballerie X
影响因子：
4
作者：
Sadlish, Heather;Galicia-Vazquez, Gabriela;Paris, C. Gregory;Aust, Thomas;Bhullar, Bhupinder;Chang, Lena;Helliwell, Stephen B.;Hoepfner, Dominic;Knapp, Britta;Riedl, Ralph;Roggo, Silvio;Schuierer, Sven;Studer, Christian;Porco, John A., Jr.;Pelletier, Jerry;Movva, N. Rao
通讯作者：
Movva, N. Rao
影响因子：
11.2
作者：
Pelletier J;Graff J;Ruggero D;Sonenberg N
通讯作者：
Sonenberg N
影响因子：
64.8
作者：
MUTHUKRISHNAN, S;BOTH, GW;SHATKIN, AJ
通讯作者：
SHATKIN, AJ
影响因子：
25.7
作者：
Ploen, Daniela;Hafirassou, Mohamed Lamine;Hildt, Eberhard
通讯作者：
Hildt, Eberhard