Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells.
Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells.
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DOI：
10.1016/j.stemcr.2017.08.004
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发表时间：
2017-10-10
影响因子：
5.9
通讯作者：
Chan YS
中科院分区：
文献类型：
--
作者：
Cai S;Tsui YP;Tam KW;Shea GK;Chang RS;Ao Q;Shum DK;Chan YS
Our ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquired phenotype and the functional capacity to myelinate axons. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SC-like cells. Co-culture of the SC-like cells with embryonic dorsal root ganglion neurons facilitated contact-mediated signaling that accomplished the switch to fate-committed SCs. Microarray analysis and in vitro myelination provided evidence that the human BMSC-derived SCs were functionally mature. This was reinforced by repair and myelination phenotypes observable in vivo with the derived SCs seeded into a nerve guide as an implant across a critical gap in a rat model of sciatic nerve injury. A protocol for in vitro derivation of fate-committed SCs from human BMSCs The derived human SCs were functionally capable of myelination in vitro The derived human SCs guided axonal regrowth and formed compact myelin in vivo Ying-Shing Chan and colleagues demonstrate that the human bone marrow harbors neuro-ectodermal progenitors that can be enriched, expanded, and directed to differentiate into functionally mature, fate-committed SCs. This work holds promise for further development into an autologous cell source for implantation as a treatment strategy for nerve injuries or peripheral neuropathies.
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6.6
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通讯作者：
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影响因子：
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从骨髓基质细胞衍生出临床适用的雪旺细胞，用于神经修复和再生。
影响因子：
3
作者：
Cai, Sa;Shea, Graham K. H.;Shum, Daisy K. Y.
通讯作者：
Shum, Daisy K. Y.