Identification of a phospholipase C beta subtype in rat taste cells.
Identification of a phospholipase C beta subtype in rat taste cells.
复制标题
大鼠味觉细胞中磷脂酶 C β 亚型的鉴定。
DOI：
10.1016/s0171-9335(98)80114-3
复制
发表时间：
1998
影响因子：
6.6
通讯作者：
H. Breer
中科院分区：
文献类型：
--
作者：
P. Rössler;Christine Kroner;Joachim Freitag;Johannes Noe;H. Breer
从大鼠轮廓乳头中克隆了一种新的磷脂酶C（PLC）亚型，并鉴定为与人PLC β2最接近的亚型。北方印迹分析表明，该基因仅在感觉舌组织中表达，而在非味觉舌组织及其它组织中均未表达。原位杂交显示轮廓乳头味觉感受器细胞的一个子集被特异性标记。通过监测苯甲酸地那铵诱导的circumvallate制剂中第二信使反应的生化分析，发现这种PLC β亚型在味觉信号转导中的功能参与。这种苦味剂引起1，4，5-三磷酸肌醇水平的快速和短暂增加;这种反应被PLC的有效抑制剂U 73122和PLC β2特异性抗体阻断。这些数据表明，磷脂酶C β 2亚型介导苯甲酸地那铵诱导的轮廓乳头味觉细胞的第二信使反应。
From rat circumvallate papillae a novel phospholipase C (PLC) subtype has been cloned and identified as most closely related to human PLC β2. The corresponding mRNA was only detected in sensory lingual tissue but not in non-taste lingual tissue or any other tissues examined by Northern blot analysis. In situ hybridization revealed that a subset of taste receptor cells of circumvallate papillae was specifically labeled. A functional involvement of this PLC β subtype in taste signal transduction emerged from biochemical analysis monitoring the second messenger response in circumvallate preparations induced by denatonium benzoate. This bitter agent elicited a rapid and transient increase of the inositol 1,4,5-trisphosphate level; this response was blocked by U73122, a potent inhibitor of PLC, and by PLC β2-specific antibodies. These data indicate that a phospholipase C β2isoform mediates a denatonium benzoate-induced second messenger response of taste sensory cells in the circumvallate papillae.
DOI：
10.1016/s0021-9258(18)41962-x
发表时间：
1992-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
C. Lee;D. Park;Dianqing Wu;S. Rhee;Melvin I. Simon
通讯作者：
C. Lee;D. Park;Dianqing Wu;S. Rhee;Melvin I. Simon
DOI：
10.1073/pnas.87.19.7395
发表时间：
1990-10-01
影响因子：
11.1
作者：
HWANG, PM;VERMA, A;SNYDER, SH
通讯作者：
SNYDER, SH