Wnt5a inhibits the proliferation and melanogenesis of melanocytes.
Wnt5a inhibits the proliferation and melanogenesis of melanocytes.
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Wnt5a 抑制黑素细胞的增殖和黑素生成
DOI：
10.7150/ijms.5664
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发表时间：
2013
影响因子：
3.6
通讯作者：
Guo H
中科院分区：
文献类型：
--
作者：
Zhang J;Li Y;Wu Y;Yang T;Yang K;Wang R;Yang J;Guo H
Wnt 5a是一种非经典的Wnt分子，已被证明参与多种发育过程和细胞功能。在本研究中，我们使用“melan-a”细胞作为细胞模型来研究Wnt 5a对黑素细胞增殖和黑素生成的影响，并阐明所涉及的可能机制。我们用重组Wnt 5a腺病毒感染melan-a细胞以表达Wnt 5a蛋白并模拟Wnt 5a加工环境。MTT法和BrdU掺入法显示Wnt 5a对melan-a细胞的增殖有明显的抑制作用。黑色素含量和酪氨酸酶活性测定表明Wnt 5a是黑色素合成的抑制剂。RT-PCR和Western blot结果表明，该抑制作用依赖于非经典Wnt/Ror 2通路的激活，而通过抑制经典Wnt通路实现。上述结果为Wnt 5a及其相关信号在黑素细胞稳态中的作用提供了新的见解。
Wnt5a, which is a noncanonical Wnt molecule, has been shown to be involved in a variety of developmental processes and cellular functions. In this study, we used “melan-a” cells as a cell model to investigate the effects of Wnt5a on melanocyte proliferation and melanogenesis, and to elucidate the possible mechanisms involved. We infected melan-a cells with recombinant Wnt5a adenoviruses to express Wnt5a protein and to simulate the Wnt5a processing environment. MTT assay and BrdU incorporation assay revealed that Wnt5a significantly inhibited the proliferation of melan-a cells. Melanin content and tyrosinase activity assays showed that Wnt5a was an inhibitor of melanin synthesis. Furthermore, RT-PCR and Western blot showed that this suppressive effect depended on noncanonical Wnt/Ror2 pathway activation and accessed the inhibition of the canonical Wnt pathway. The above results provided a novel insight into the role of Wnt5a and its related signaling in melanocyte homeostasis.
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DOI：
10.1038/jid.2008.433
发表时间：
2009-07
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
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影响因子：
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DOI：
10.1073/pnas.0909409106
发表时间：
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影响因子：
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作者：
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