Extracellular matrix protein composition dynamically changes during murine forelimb development.
Extracellular matrix protein composition dynamically changes during murine forelimb development.
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DOI：
10.1016/j.isci.2024.108838
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发表时间：
2024-02-16
期刊：
影响因子：
5.8
通讯作者：
Calve, Sarah
中科院分区：
文献类型：
--
作者：
Jacobson, Kathryn R.;Saleh, Aya M.;Lipp, Sarah N.;Tian, Chengzhe;Watson, Audrey R.;Luetkemeyer, Callan M.;Ocken, Alexander R.;Spencer, Sabrina L.;Kinzer-Ursem, Tamara L.;Calve, Sarah
细胞外基质（ECM）是多细胞生物不可或缺的一部分，它连接着不同的细胞层和组织类型。在形态发生和生长过程中，组织会经历重大的重组。虽然直观上ECM也会同步重塑，但关于其成分和结构在发育过程中如何变化，我们所知甚少。在此，我们利用组织分级分离、生物正交非天然氨基酸标记和质谱技术，对小鼠前肢在附肢肌肉骨骼形态发生期间（胚胎第11.5 - 14.5天）的ECM蛋白动态变化进行了量化分析。我们的分析表明，胚胎前肢中的ECM蛋白（基质组）成分随发育和生长而变化，与其他发育中的器官（如大脑）不同，并且在疾病模型（小鼠成骨不全症）中也有所改变。此外，我们还通过免疫组织化学方法评估了野生型胚胎及出生后肌肉骨骼系统中特定基质组的组织分布。这一研究成果将为未来探究基质组在复杂组织发育过程中的作用提供指导。
- ECM成分在前肢发育过程中发生变化，与其他器官不同
- 转录组学无法预测ECM蛋白动态变化和结构
- 体内代谢标记揭示前肢中新生ECM的动态变化
自然科学；生物科学；发育生物学
The extracellular matrix (ECM) is an integral part of multicellular organisms, connecting different cell layers and tissue types. During morphogenesis and growth, tissues undergo substantial reorganization. While it is intuitive that the ECM remodels in concert, little is known regarding how matrix composition and organization change during development. Here, we quantified ECM protein dynamics in the murine forelimb during appendicular musculoskeletal morphogenesis (embryonic days 11.5–14.5) using tissue fractionation, bioorthogonal non-canonical amino acid tagging, and mass spectrometry. Our analyses indicated that ECM protein (matrisome) composition in the embryonic forelimb changed as a function of development and growth, was distinct from other developing organs (brain), and was altered in a model of disease (osteogenesis imperfecta murine). Additionally, the tissue distribution for select matrisome was assessed via immunohistochemistry in the wild-type embryonic and postnatal musculoskeletal system. This resource will guide future research investigating the role of the matrisome during complex tissue development. ECM composition changed during forelimb development, distinct from other organs ECM protein dynamics and organization cannot be predicted by transcriptomics In vivo metabolic labeling identified dynamics of nascent ECM in forelimbs Natural sciences; Biological sciences; Developmental biology
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影响因子：
14.9
作者：
Gene Ontology Consortium
通讯作者：
Gene Ontology Consortium
影响因子：
3.5
作者：
Chaudhry, SS;Gazzard, J;Dixon, MJ
通讯作者：
Dixon, MJ
影响因子：
14.8
作者：
Alvarez-Castelao, Beatriz;Schanzenbaecher, Christoph T.;Schuman, Erin M.
通讯作者：
Schuman, Erin M.
影响因子：
2
作者：
Can, Tugba;Faas, Laura;Blanco, Gonzalo
通讯作者：
Blanco, Gonzalo
DOI：
10.1161/atvbaha.117.310562
发表时间：
2018-07
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Fava M;Barallobre-Barreiro J;Mayr U;Lu R;Didangelos A;Baig F;Lynch M;Catibog N;Joshi A;Barwari T;Yin X;Jahangiri M;Mayr M
通讯作者：
Mayr M