Effects of Different Conditions of Retinoic Acid Treatment on Expression of MK Gene, which is Transiently Activated during Differentiation of Embryonal Carcinoma Cells 1
Effects of Different Conditions of Retinoic Acid Treatment on Expression of MK Gene, which is Transiently Activated during Differentiation of Embryonal Carcinoma Cells 1
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不同视黄酸处理条件对胚胎癌细胞分化过程中瞬时激活的MK基因表达的影响1
DOI：
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
T. Muramatsu
中科院分区：
文献类型：
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作者：
Ruo;H. Muramatsu;T. Muramatsu
当HM-1胚胎癌（EC）细胞的聚集体用视黄酸处理2天以诱导分化为神经细胞、成肌细胞和胚外内胚层细胞时，MK基因强烈表达。抑制神经细胞分化或胚外内胚层细胞分化的条件对MK基因表达影响不大。MK RNA的最高水平检测2天后开始维甲酸治疗，当细胞形态学上无法区分未分化的EC细胞。因此，MK基因似乎在分化中的EC细胞中表达，而与分化方向无关。在稀疏培养的HM-1细胞中MK基因表达的程度与维甲酸的浓度相关，特别是在10 - 8和10 - 7 M之间。当维甲酸处理1天后终止时，MK RNA的量开始减少。这两个结果与维甲酸与受体复合直接参与MK基因表达的观点一致。
MK gene was intensely expressed, when aggregates of HM‐1 embryonal carcinoma (EC) cells were treated with retinoic acid for 2 days to induce the differntiation to nerve cells, myoblasts and extraembryonic endoderm cells. The conditions inhibiting nerve cell diffrentiation or extraembryonic endoderm cell differentiation affected MK gene expression only slightly. The maximum level of MK RNA was detected 2 days after initiation of retionic acid treatment, when cells were morphologically indistinguishable from undifferentiated EC cells. Thus, MK gene appears to be expressed in differentiating EC cells irrespective of the direction of differentiation. The degree of MK gene expression in sparsely cultured HM‐1 cells correlated with the concentration of retinoic acid, especially between 10‐8 and 10‐7 M. When retinoic acid treatment was terminated after 1 day, the amount of MK RNA started to decrease. These two results are consistent with the view that retionic acid complexed with the receptor is directly involved in expression of MK gene.
类维生素A受体反义DNA抑制恶性角质形成细胞中的碱性磷酸酶诱导和克隆形成。
DOI：
10.1073/pnas.86.14.5590
发表时间：
1989
影响因子：
11.1
作者：
Cope,FO;Wille,JJ
通讯作者：
Wille,JJ
DOI：
10.1073/pnas.85.2.329
发表时间：
1988
影响因子：
11.1
作者：
G. Larosa;L. Gudas
通讯作者：
G. Larosa;L. Gudas