Retinoid receptor antisense DNAs inhibit alkaline phosphatase induction and clonogenicity in malignant keratinocytes.
Retinoid receptor antisense DNAs inhibit alkaline phosphatase induction and clonogenicity in malignant keratinocytes.
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类维生素A受体反义DNA抑制恶性角质形成细胞中的碱性磷酸酶诱导和克隆形成。
DOI：
10.1073/pnas.86.14.5590
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发表时间：
1989
影响因子：
11.1
通讯作者：
Wille,JJ
中科院分区：
文献类型：
--
作者：
Cope,FO;Wille,JJ
合成了与人细胞视黄醇结合蛋白I(CRBP)和人核维甲酸受体α(HnRAR)相对应的反义寡核苷酸[寡(DN)S]。人恶性角质形成细胞暴露于这些寡核苷酸(DN)后，S以浓度和时间依赖的方式显著降低胞浆cRBP和hnRAR水平。此外，30微米针对cRBP或hnRAR的反义寡核苷酸(DN)可阻断视黄醇对这些细胞中碱性磷酸酶的诱导作用，但30微米针对cRBP的正义寡核苷酸(DN)不能阻断这一作用。反义寡核苷酸(DN)处理可导致细胞变圆、细胞间黏附丧失以及细胞与基质的黏附。相反，用抗细胞动剂细胞松弛素B或细胞抑制浓度的叠氮化钠处理细胞并不能减少核提取液中的细胞质cRBP或hnRAR，即使在细胞形态中观察到反义寡核苷酸(DN)样的变化。用20-40微米的反义寡核苷酸(DN)处理细胞超过2.75小时，也会导致克隆形成能力的丧失。这些结果表明，维甲酸的胞浆受体和核受体在维甲酸信号反应的转导中都是重要的，维甲酸信号反应对细胞的生长和分化至关重要。我们的发现还表明，由维甲酸及其受体指定的特定基因可能解释了维生素A化合物的多效性。
Antisense oligodeoxynucleotides [oligo(dN)s] corresponding to human cellular retinol-binding protein I (cRBP) and human nuclear retinoic acid receptor alpha (hnRAR) were synthesized. Exposure of human malignant keratinocytes to these oligo(dN)s significantly attenuated the level of cytoplasmic cRBP and hnRAR in a concentration- and time-dependent manner. Further, the induction of alkaline phosphatase by retinol in these cells was blocked by treatment with 30 microM antisense oligo(dN) to cRBP or hnRAR but not by 30 microM of sense oligo(dN) to cRBP. Antisense oligo(dN) treatments concomitantly induced cell rounding, loss of cell-cell attachment, and cell adhesion to the substratum. By contrast, treatment of cells with an anticytokinetic agent, cytochalasin B, or with a cytostatic concentration of sodium azide failed to reduce cytoplasmic cRBP or hnRAR from nuclear extracts, even though antisense oligo(dN)-like changes in cell morphology were observed. Treatment of the cells for greater than 2.75 hr with 20-40 microM of either antisense oligo(dN) also led to the loss of clonogenic potential. These results show that both cytoplasmic and nuclear receptors for retinoids are important in the transduction of a retinoid signal response critical to cellular growth and differentiation. Our findings also suggest that defined genes, which are specified by retinoids and their receptors, may account for the pleiotropic effect of vitamin A compounds.
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系统性红斑狼疮患者外周血中人类 B 细胞集落的生长。
影响因子：
27.4
作者：
A. Fay;S. Price;A. Trudgett;J. Mccrea;M. Haire;M. W. Boyd;T. Mcneill
通讯作者：
T. Mcneill
影响因子：
--
作者：
Huggins, CB
通讯作者：
Huggins, CB
上颌窦癌碱性磷酸酶Regan同工酶的免疫过氧化物酶研究。
DOI：
--
发表时间：
1986
期刊：
Oral surgery, oral medicine, and oral pathology
影响因子：
--
作者：
H. Shikata;M. Noguchi;N. Utsumi;K. Suzuki;H. Ushiyama;T. Uchida;T. Shikata;S. Iino;K. Hirano
通讯作者：
K. Hirano
使用免疫过氧化物酶和免疫金银染色程序对胎牛骨中的碱性磷酸酶进行光学显微镜定位。
DOI：
10.1177/36.3.3278058
发表时间：
1988
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
D. Morris;J. C. Randall;H. Anderson
通讯作者：
H. Anderson
电子显微细胞化学方法研究骨骺软骨磷酸酶
影响因子：
3.2
作者：
T. Matsuzawa;H. Anderson
通讯作者：
H. Anderson