The Therapeutic Effect of Shugan Xiehuo Formula in the Female Rat Model with Central Precocious Puberty.
The Therapeutic Effect of Shugan Xiehuo Formula in the Female Rat Model with Central Precocious Puberty.
复制标题
疏肝泻火方对雌性中枢性性早熟模型大鼠的治疗作用
DOI：
10.1155/2020/5916168
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Xia J
中科院分区：
文献类型：
--
作者：
Yin W;Li S;Zhang K;Xu Y;Ma D;Shi T;Xiong L;Xia J
中枢性性早熟（CPP）严重影响儿童身心健康，需要及时有效治疗。目的：研究疏肝泻火方对慢性偏头痛的治疗作用。建立雌性CPP大鼠模型，然后用亮丙瑞林和不同剂量的SXF治疗。测量性器官体积和指数。通过苏木精-伊红染色观察卵巢和子宫。测定外周血中促卵泡激素（FSH）、促黄体生成素（LH）、催乳素（PRL）和雌二醇（E2）的浓度。采用实时荧光定量逆转录聚合酶链反应（Real-Time Quantitative Reverse Transcription PCR）和免疫印迹法（Western blot）检测垂体促性腺激素释放激素（GnRH）、促性腺激素释放激素受体（GnRHR）、雌激素受体α（ERα）和G蛋白偶联受体30（GPR 30）的表达水平。免疫组化法检测下丘脑GnRH和卵巢GnRH受体的表达。SXF能降低CPP雌性大鼠双侧卵巢体积、子宫、下丘脑和垂体体积，降低CPP雌性大鼠卵巢、子宫、下丘脑和垂体指数（P < 0.05或P < 0.01）。SXF治疗抑制雌性CPP大鼠的卵泡成熟和子宫壁增厚。SXF能降低CPP雌性大鼠外周血FSH、LH、PRL和E2的浓度（P < 0.01或P < 0.001）。SXF可抑制CPP雌性大鼠垂体GnRH、GnRHR、ERα、GPR 30的表达（P < 0.05），下丘脑GnRH的表达（P < 0.01），卵巢GnRHR的表达（P < 0.001）。总之，我们的研究表明，SXF对雌性大鼠CPP有治疗作用。值得临床推广。
Central precocious puberty (CPP) severely affects children's physical and mental health and needs to be treated promptly and effectively. This article aimed to research the therapeutic effect of Shugan Xiehuo Formula (SXF) on CPP. A female CPP rat model was established and then treated with leuprolide and different doses of SXF. Sex organ volume and index were measured. Ovaries and uteri were visualized by hematoxylin-eosin staining. The concentrations of follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), and estradiol (E2) in peripheral blood were determined. The expression levels of gonadotropin-releasing hormone (GnRH), gonadotropin-releasing hormone receptor (GnRHR), estrogen receptor alpha (ERα), and G protein-coupled receptor 30 (GPR30) in the hypophysis were investigated by Real-Time Quantitative Reverse Transcription PCR and western blot. GnRH expression in the hypothalamus and GnRHR expression in the ovary were detected by immunohistochemistry. SXF reduced the volume of the bilateral ovaries, as well as the volumes of the uterus, hypothalamus, and hypophysis in the female CPP rats and diminished the index of the ovary, uterus, hypothalamus, and hypophysis in the female CPP rats (P < 0.05 or P < 0.01). SXF treatment inhibited follicle maturation and uterine wall thickening in the female CPP rats. SXF decreased the concentrations of FSH, LH, PRL, and E2 in the peripheral blood in the female CPP rats (P < 0.01 or P < 0.001). SXF suppressed the expressions of GnRH, GnRHR, ERα, and GPR30 in the hypophysis (P < 0.05), the expression of GnRH in the hypothalamus (P < 0.01), and the expression of GnRHR in the ovaries (P < 0.001) of the female CPP rats. Overall, our study revealed that SXF had therapeutic effects on CPP in female rats. This is worthy of promoting clinically.
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影响因子：
3.7
作者：
Lee HS;Yoon JS;Park KJ;Hwang JS
通讯作者：
Hwang JS
DOI：
10.1007/s40272-015-0130-8
发表时间：
2015-08
期刊：
Paediatric drugs
影响因子：
--
作者：
Chen M;Eugster EA
通讯作者：
Eugster EA
影响因子：
13.3
作者：
Skorupskaite K;George JT;Anderson RA
通讯作者：
Anderson RA
影响因子：
4.1
作者：
Eugster, Erica A.
通讯作者：
Eugster, Erica A.
影响因子：
1.4
作者：
Luis Quintanar, Jose;Diaz-Galindo, Carmen;Olvera-Sandoval, Carlos
通讯作者：
Olvera-Sandoval, Carlos