Adsorption of plasma proteins on polyethylene oxide-modified lipid bilayers studied by total internal reflection fluorescence.

Adsorption of plasma proteins on polyethylene oxide-modified lipid bilayers studied by total internal reflection fluorescence.
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通过全内反射荧光研究血浆蛋白在聚环氧乙烷修饰的脂质双层上的吸附。

DOI:
10.1016/s0142-9612(00)00004-1
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发表时间:
2000
期刊:
影响因子:
14
通讯作者:
Marchant,RE
Marchant,RE
中科院分区:
工程技术1区
文献类型:
--
作者:
Xu,Z;Marchant,RE

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采用Langmuir-Blodgett沉积法将二硬脂酰磷脂酰胆碱(DSPC)与不同摩尔百分比的聚氧化物(数平均分子量2000)接枝二硬脂酰磷脂酰乙醇胺(PEO2000-DSPE)沉积在dspe涂层的石英表面上。薄膜平衡实验揭示了PEO2000从煎饼到蘑菇、从蘑菇到刷子的结构转变。利用本征全内反射荧光研究了1%血小板贫血浆(PPP)蛋白在脂质双分子层上的吸附动力学。与石英底物相比,所有支持的脂质双分子层的吸附血浆蛋白都减少了一个数量级以上。混合双分子层中PEO2000-DSPE浓度的增加使双分子层上吸附蛋白的数量略有增加。
Distearoylphophatidylcholine (DSPC) mixed with various mole percentages of polyethylene oxide (number average molecular weight 2000)-grafted distearoylphosphatidylethanolamine (PEO2000-DSPE) were deposited on DSPE-coated quartz surfaces by the Langmuir–Blodgett deposition. Structural transitions in PEO2000 from pancake to mushroom, and from mushroom to brush conformations were revealed from film balance experiments. Adsorption kinetics of proteins from 1% platelet-poor plasma (PPP) on the supported lipid bilayers were studied using intrinsic total internal reflection fluorescence. All the supported lipid bilayers exhibited over a magnitude reduction in adsorbed plasma proteins, compared with the quartz substrate. The increase of PEO2000-DSPE density in the mixed bilayers slightly increases the amount of adsorbed proteins on the bilayers.
与血液-物质相互作用相关的固溶体界面的蛋白质吸附
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