Investigating chitin deacetylation and chitosan hydrolysis during vegetative growth in Magnaporthe oryzae.
Investigating chitin deacetylation and chitosan hydrolysis during vegetative growth in Magnaporthe oryzae.
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研究麦糖蛋白蛋白酶生长过程中的几丁质脱乙酰化和壳聚糖水解。
DOI：
10.1111/cmi.12743
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发表时间：
2017-09
影响因子：
3.4
通讯作者：
Gurr SJ
中科院分区：
文献类型：
--
作者：
Geoghegan IA;Gurr SJ
甲壳素脱乙酰化导致壳聚糖的形成，壳聚糖是β 1，4-连接的葡萄糖胺的聚合物。已知壳聚糖在许多真菌物种的细胞壁中具有重要功能，但其在菌丝生长期间的作用尚未被研究。在这项研究中，我们的特点是在丝状植物病原菌Magnaporthe nerve营养菌丝生长过程中的几丁质脱乙酰化的作用。我们发现，壳聚糖定位于营养菌丝的隔膜和侧细胞壁，并确定2几丁质脱乙酰酶在营养生长过程中表达-CDA 1和CDA 4。缺失菌株和荧光蛋白融合表明，CDA 1是必要的成熟菌丝在菌落内部的隔膜和侧细胞壁几丁质脱乙酰，而CDA 4脱乙酰几丁质在菌丝在菌落边缘。然而，尽管Δ cda 1菌株对细胞壁水解更具抗性，但在缺失菌株中生长和致病性发展不受影响。壳聚糖水解的作用也进行了研究。一个编码壳聚糖酶（CSN）的单一基因被发现在M. martinus中，并发现在营养生长过程中表达。然而，壳聚糖定位，营养生长和致病性发展不受影响的CSN缺失菌株，使这种酶的作用不清楚。
Chitin deacetylation results in the formation of chitosan, a polymer of β1,4‐linked glucosamine. Chitosan is known to have important functions in the cell walls of a number of fungal species, but its role during hyphal growth has not yet been investigated. In this study, we have characterized the role of chitin deacetylation during vegetative hyphal growth in the filamentous phytopathogen Magnaporthe oryzae. We found that chitosan localizes to the septa and lateral cell walls of vegetative hyphae and identified 2 chitin deacetylases expressed during vegetative growth—CDA1 and CDA4. Deletion strains and fluorescent protein fusions demonstrated that CDA1 is necessary for chitin deacetylation in the septa and lateral cell walls of mature hyphae in colony interiors, whereas CDA4 deacetylates chitin in the hyphae at colony margins. However, although the Δcda1 strain was more resistant to cell wall hydrolysis, growth and pathogenic development were otherwise unaffected in the deletion strains. The role of chitosan hydrolysis was also investigated. A single gene encoding a putative chitosanase (CSN) was discovered in M. oryzae and found to be expressed during vegetative growth. However, chitosan localization, vegetative growth, and pathogenic development were unaffected in a CSN deletion strain, rendering the role of this enzyme unclear.
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影响因子：
3.7
作者：
Che Omar S;Bentley MA;Morieri G;Preston GM;Gurr SJ
通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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