Interrogating nitritation at a molecular level: Understanding the potential influence of Nitrobacter spp.
Interrogating nitritation at a molecular level: Understanding the potential influence of Nitrobacter spp.
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在分子水平上探讨亚硝化：了解硝化细菌的潜在影响。
DOI：
10.1016/j.watres.2022.119074
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发表时间：
2022
期刊：
影响因子：
12.8
通讯作者：
Coats, Erik R.
中科院分区：
文献类型：
--
作者：
Smoot, Lindsey;Mellin, Jason;Brinkman, Cynthia K.;Popova, Inna;Coats, Erik R.
水资源回收设施（WRRF）越来越多地必须最大限度地去除氮和磷，但同时面临着减少能源使用和环境足迹的挑战。特别是，生物营养物去除（BNR），其目标是去除磷和氮，表现出很大的能量需求。然而，实现NH3部分氧化为NO2（亚硝化）的BNR工艺可以减少能源需求，并具有二次环境排放效益。对进行亚硝化和硝化的实验室规模系统进行了研究，以更好地了解在真实的废水上培养的混合微生物聚生体如何能够维持亚硝化。BNR配置实现了64- 82%的亚硝酸盐积累率，具有优异的整体出水质量。应用系统发育学、转录组学和代谢组学方法，结合过程监测，结果表明，部分亚硝化可以通过以下组合来诱导：（1）采用基于氨的曝气控制，氨设定值为2.3 mgN/L，（2）维持好氧期DO为1.0-2.0 mg/L，（3）维持好氧期DO为1.0-2.0 mg/L，（4）维持好氧期DO为1.0-2.0 mg/L。（3）BNR在缺氧后运行，并与强化生物除磷（EBPR）相结合。尽管存在亚硝化菌，但亚硝酸盐氧化还原酶必须是功能受损或结构不完整的。总的来说，这项研究证明了在宏观和分子水平上询问混合微生物财团以探索独特的代谢反应（如亚硝酸盐）的价值。
Water resource recovery facilities (WRRFs) increasingly must maximize nitrogen and phosphorus removal, but concurrently face challenges to reduce their energy usage and environmental footprint. In particular, biological nutrient removal (BNR), which targets removal of phosphorus and nitrogen, exhibits a large energy demand. However, a BNR process achieving partial oxidation of NH3to NO2(nitritation) could reduce energy demands, with secondary environmental emission benefits. Research was conducted on bench-scale systems performing nitritation and nitrification to better understand how mixed microbial consortia, cultured on real wastewater, can sustain nitritation. BNR configurations achieved nitrite accumulation ratios of 64–82%, with excellent overall effluent quality. Applying phylogenetic, transcriptomic, and metabolomic methods, coupled with process monitoring, results indicate that partial nitritation may be induced through a combination of: (1) Employing ammonia-based aeration control, with an ammonia setpoint of 2, 3 mgN/L; (2) Maintaining an aerobic period DO of 1.0–2.0 mg/L; and (3) Operating BNR post-anoxically, integrated within enhanced biological phosphorus removal (EBPR). Significant nitritation was achieved despite the presenceNitrobacterspp., but nitrite oxidoreductase must be functionally impaired or structurally incomplete. Overall, this research demonstrated the value of interrogating a mixed microbial consortia at a macro and molecular level to explore unique metabolic responses such as nitritation.
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