Correlative association between N-methyl-D-aspartate receptor-mediated expression of period genes in the suprachiasmatic nucleus and phase shifts in behavior with photic entrainment of clock in hamsters.
Correlative association between N-methyl-D-aspartate receptor-mediated expression of period genes in the suprachiasmatic nucleus and phase shifts in behavior with photic entrainment of clock in hamsters.
复制标题
仓鼠视交叉上核中 N-甲基-D-天冬氨酸受体介导的周期基因表达与时钟光夹带行为相移之间的相关性。
作者：
T. Moriya;K. Horikawa;M. Akiyama;S. Shibata
由于Per 基因的快速诱导与生物钟的光夹带有关，我们检查了仓鼠视交叉上核（SCN）中N-甲基-D-天冬氨酸（NMDA）受体是否参与Per 基因的光诱导。原位杂交观察显示，主观夜间早期[昼夜节律时间（CT）13.5]或主观深夜（CT20）期间的光引起SCN中Per1和Per2的诱导，但不诱导Per3 mRNA。 CT13.5 处的 Per mRNA 光诱导尤其在腹外侧 SCN 中观察到，而 CT20 处的光诱导从腹外侧到背侧 SCN 更为广泛。非竞争性 NMDA 受体拮抗剂 +MK801，剂量依赖性 (0. 1-5.0 mg/kg) 仅抑制 SCN 中 CT13.5 或 CT20 光诱导的 Per1 和 Per2 mRNA 的腹外侧部分。 +MK801 对 Per mRNA 的抑制作用与该化合物对 CT13.5 和 CT20 光相移的减弱作用密切相关。竞争性 NMDA 受体拮抗剂 D-2-氨基-5-磷酸戊酸 (D-APV) 也对光 (CT20) 诱导的腹外侧 SCN 中 Per1 和 Per2 mRNA 表达表现出抑制作用。此外，将 NMDA 局部注射到 SCN 中导致 SCN 中 Per1 和 Per2 mRNA 的诱导。 NMDA 受体中，NR2B 和 NR2C mRNA 分别在腹外侧和背侧 SCN 中表达。这些结果表明，NMDA 受体的激活是 SCN 中 Per1 和 Per2 转录本光诱导的关键步骤，这与光行为夹带有关。
Because the rapid induction of Period (Per) genes is associated with the photic entrainment of the biological clock, we examined whether N-methyl-D-aspartate (NMDA) receptors were involved in the photic induction of Per genes in the hamster suprachiasmatic nucleus (SCN). In situ hybridization observation revealed that light during the early subjective night [circadian time (CT) 13.5] or the late subjective night (CT20) caused an induction of Per1 and Per2 but not Per3 mRNA in the SCN. Photic induction of Per mRNA at CT13.5 was observed especially in the ventrolateral SCN, whereas that at CT20 was more widespread from the ventrolateral to the dorsal SCN. A noncompetitive NMDA receptor antagonist, +MK801, dose-dependently (0. 1-5.0 mg/kg) suppressed only the ventrolateral part of Per1 and Per2 mRNA induction by light at CT13.5 or CT20 in the SCN. The suppressive effects of +MK801 on Per mRNA strongly correlated with the attenuating action of this compound on phase shifts by light at both CT13.5 and CT20. A competitive NMDA receptor antagonist, D-2-amino-5-phosphonovalerate (D-APV), also exhibited inhibitory actions on light (CT20)-induced Per1 and Per2 mRNA expression in the ventrolateral SCN. Furthermore, local injection of NMDA into the SCN resulted in the induction of Per1 and Per2 mRNA in the SCN. Among NMDA receptors, NR2B and NR2C mRNA were expressed in the ventrolateral and dorsal SCN, respectively. These results suggest that the activation of NMDA receptor is a critical step for photic induction of Per1 and Per2 transcripts in the SCN, which are linked to a photic behavioral entrainment.
DOI：
10.1016/0169-328x(94)00212-w
发表时间：
1995-02-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
OHARA, BF;ANDRETIC, R;KILDUFF, TS
通讯作者：
KILDUFF, TS
影响因子：
56.9
作者：
RALPH, MR;FOSTER, RG;MENAKER, M
通讯作者：
MENAKER, M